| IED ID | IndEnz0008000376 |
| Enzyme Type ID | cellulase000376 |
| Protein Name |
Endoglucanase 3 EC 3.2.1.4 Cellulase 3 Endo-1,4-beta-glucanase 3 |
| Gene Name | CMC3 |
| Organism | Humicola insolens (Soft-rot fungus) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Sordariomycetidae Sordariales Chaetomiaceae Humicola Humicola insolens (Soft-rot fungus) |
| Enzyme Sequence | MKHSVLAGLFATGALAQGGAWQQCGGVGFSGSTSCVSGYTCVYLNDWYSQCQPQPTTLRTTTTPGATSTTRSAPAATSTTPAKGKFKWFGINQSCAEFGKGEYPGLWGKHFTFPSTSSIQTHINDGFNMFRVAFSMERLAPNQLNAAFDANYLRNLTETVNFITGKGKYAMLDPHNFGRYYERIITDKAAFASFFTKLATHFASNPLVVFDTNNEYHDMDQQLVFDLNQAAIDAIRAAGATSQYIMVEGNSWTGAWTWNVTNNNLAALRDPENKLVYQMHQYLDSDGSGTSTACVSTQVGLQRVIGATNWLRQNGKVGLLGEFAGGANSVCQQAIEGMLTHLQENSDVWTGALWWAGGPWWGDYIYSFEPPSGIGYTYYNSLLKKYVP |
| Enzyme Length | 388 |
| Uniprot Accession Number | Q12624 |
| Absorption | |
| Active Site | ACT_SITE 215; /note=Proton donor; /evidence=ECO:0000250; ACT_SITE 322; /note=Nucleophile; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; |
| DNA Binding | |
| EC Number | 3.2.1.4 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (1); Disulfide bond (2); Domain (1); Glycosylation (3); Region (3); Sequence conflict (2); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Cellulose degradation;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..16; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 42,564 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |