| IED ID | IndEnz0008000386 |
| Enzyme Type ID | cellulase000386 |
| Protein Name |
Mannan endo-1,4-beta-mannosidase A EC 3.2.1.78 1,4-beta-D-mannan mannanohydrolase A Beta-mannanase A |
| Gene Name | MANA |
| Organism | Piromyces sp. |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Fungi incertae sedis Chytridiomycota Chytridiomycota incertae sedis Neocallimastigomycetes Neocallimastigales Neocallimastigaceae Piromyces unclassified Piromyces Piromyces sp. |
| Enzyme Sequence | MKSLNVILTLLSLIISVLSKKVYYEAEDGKLNGITVFKELSGFSGKGYVGRFENPGNSVTVTVDAPATGMYDLSIIYCANMGQKINSLTVNDQSVGDITFTENTKFETKDVGAVYLNKGKNTIGLVSSWGWMWVDAFVINDAPNAAKDVSSKLNPTLVNPKAIPAAKKLYDFLKTNYGKRILSGQVGAAGQAGDEGQEIQRIQKATGKLPAVWNMDFIFESNDCTWRPQNPDITEMAINWWKKYEGKGIMAAQWHWNIAGKTGDFAFYSKDTTFNLENAVTEGTWEYEKIIKDIDRVSGHIKKLQAVNMPLIWRPLHENNGDWFWWGNNPKACAKLWKILYERMVNYHGLNNLIWLWNGNNDANTPVDYIDIIGVDIYANDHGPQTTAYNTHFDFYGGKKMVVLSENGRIPDIQQCVDQDVWWGYFQTWNSEFILQDSYHTDAQLKEYFNHKTVMNMDELPSFNVDSYNGDSGSSHNGNSESNSNTGNSDECWSINLGYPCCIGDYVVTTDENGDWGVENNEWCGIVHKSCWSEPLGYPCCVGNTVISADESGDWGVENNEWCGIVHKSCWAEFLGYPCCVGNTVISTDEFGDWGVENDDWCGILN |
| Enzyme Length | 606 |
| Uniprot Accession Number | P55296 |
| Absorption | |
| Active Site | ACT_SITE 318; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01100; ACT_SITE 406; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU01100 |
| Activity Regulation | |
| Binding Site | BINDING 285; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 323; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 378; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 493; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.; EC=3.2.1.78; |
| DNA Binding | |
| EC Number | 3.2.1.78 |
| Enzyme Function | FUNCTION: Hydrolyzes 1,4-beta linked polysaccharide backbones of mannans, one of the major hemicellulose components in hardwoods and softwoods. Shows very high activity against mannohexaose but not against mannopentaose and smaller mannooligosaccharides. The major products released from mannooligosaccharide hydrolysis are mannose and mannobiose. The reiterated 40 AA domain is involved in binding the cellulase-hemicellulase complex. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Binding site (4); Chain (1); Domain (5); Region (1); Signal peptide (1) |
| Keywords | Glycosidase;Hydrolase;Repeat;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 68,055 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |