| IED ID | IndEnz0008000390 |
| Enzyme Type ID | cellulase000390 |
| Protein Name |
Probable mannan endo-1,4-beta-mannosidase A EC 3.2.1.78 Endo-beta-1,4-mannanase A |
| Gene Name | manA man1 AO090010000122 |
| Organism | Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus oryzae (Yellow koji mold) Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) |
| Enzyme Sequence | MKLNPSLLTAAGLVSAQLASALPQASSSTVSPSPSPSATPGSFVTTSGLNFVIDGKTGYFAGSNSYWIGFQKNNDDVDLVFSHLQESGLKILRVWGFNDVNQKPTDGSVYYHLLADGTATVNEGEDGLQRLDYVVSSAEKHGIKLIINFVNFWDDYGGINAYVKAFGGSKEGFYTNDAMQAAYRAYIKAVISRYSDSTAIFAWELANEPRCQGCETTVLYNWIESTSQYIKSLDSKHLVCIGDEGFGLDTGSDGSYPYQYSEGSDFAKNLAIPTIDFGTFHLYPSSWGTTNDWGNGWVTSHGAACKAAGKPCLFEEYGVTSDHCAVEKPWQNTALNTTAISGDLYWQYGDQLSGGPSPDDGNTFYYGTDDFKCLVTDHIAAINSRK |
| Enzyme Length | 386 |
| Uniprot Accession Number | Q2TXJ2 |
| Absorption | |
| Active Site | ACT_SITE 208; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:Q99036; ACT_SITE 316; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q99036 |
| Activity Regulation | |
| Binding Site | BINDING 95; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 207; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 283; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 346; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.; EC=3.2.1.78; |
| DNA Binding | |
| EC Number | 3.2.1.78 |
| Enzyme Function | FUNCTION: Endo-1,4-mannanase, a crucial enzyme for depolymerization of seed galactomannans and wood galactoglucomannans. {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Binding site (4); Chain (1); Glycosylation (1); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Glycoprotein;Glycosidase;Hydrolase;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..21; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 41,906 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |