| IED ID | IndEnz0008000393 |
| Enzyme Type ID | cellulase000393 |
| Protein Name |
Endoglucanase 9 EC 3.2.1.4 Cellulase 3 AtCEL3 Endo-1,4-beta glucanase 9 |
| Gene Name | CEL3 At1g71380 F26A9.24 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MTSLFFFVLLFSSLLISNGDANPNYKEALSKSLLFFQGQRSGPLPRGQQISWRASSGLSDGSAAHVDLTGGYYDAGDNVKFNLPMAFTTTMLSWSALEYGKRMGPELENARVNIRWATDYLLKCARATPGKLYVGVGDPNVDHKCWERPEDMDTPRTVYSVSASNPGSDVAAETAAALAAASMVFRKVDSKYSRLLLATAKDVMQFAIQYQGAYSDSLSSSVCPFYCSYSGYKDELMWGASWLLRATNNPYYANFIKSLGGGDQPDIFSWDNKYAGAYVLLSRRALLNKDSNFEQYKQAAENFICKILPDSPSSSTQYTQGGLMYKLPQSNLQYVTSITFLLTTYAKYMKATKHTFNCGSSVIVPNALISLSKRQVDYILGDNPIKMSYMVGFSSNFPKRIHHRASSLPSHALRSQSLGCNGGFQSFYTQNPNPNILTGAIVGGPNQNDGYPDQRDDYSHAEPATYINAAFVGPLAYFAAGRST |
| Enzyme Length | 484 |
| Uniprot Accession Number | Q9C9H5 |
| Absorption | |
| Active Site | ACT_SITE 77; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10140; ACT_SITE 402; /evidence=ECO:0000255|PROSITE-ProRule:PRU10059; ACT_SITE 453; /evidence=ECO:0000255|PROSITE-ProRule:PRU10060; ACT_SITE 462; /evidence=ECO:0000255|PROSITE-ProRule:PRU10060 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; |
| DNA Binding | |
| EC Number | 3.2.1.4 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (4); Chain (1); Frameshift (1); Sequence conflict (3); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Cell wall;Cell wall biogenesis/degradation;Cellulose degradation;Direct protein sequencing;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000269|PubMed:9188482}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..21; /evidence=ECO:0000269|PubMed:9188482 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 14576160; 14595688; 15769804; 16287169; 16367961; 16648215; 17056621; 17317660; 17581762; 17687051; 18775970; 20706207; 21166475; 24045021; 25600937; |
| Motif | |
| Gene Encoded By | |
| Mass | 53,274 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.2.1.4; |