| IED ID | IndEnz0008000394 |
| Enzyme Type ID | cellulase000394 |
| Protein Name |
Endoglucanase 9 EC 3.2.1.4 Endo-1,4-beta glucanase 9 OsCel9D OsGLU1 |
| Gene Name | GLU1 Os03g0329500 LOC_Os03g21210 |
| Organism | Oryza sativa subsp. japonica (Rice) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. japonica (Rice) |
| Enzyme Sequence | MSMYGRDPWGGPLEICHDSATDDDRSRNLDLDRGALSRTLDETQQSWLLAGPGDQGRKKKKYVDLGCLVVSRKLFVWTVGVLLAAAVFAGLVAGIAKAIPRHHRPPPPPDDFTVALRKALMFFNAQKSGKLPKNNNVHWRGNSCMKDGLSDPAVGRSLVGGYYDAGDAVKFNFPAAFSMTLLSWSVIEYSAKYEAVGELGHIRDTIKWGADYFLKTFNSTADTIDRVVMQVGSGATSPGSTQPNDHYCWMRPEDIDYPRPVVECHACSDLAAEMAASLAAASIVFKDNKAYSQKLVHGATTLFKFARQNRGRYSAGGSDAAKFYNSTSYWDEFVWGGSWMYLATGNSSYLQLATHPKLAKHAGAYWGGPDYGVFSWDNKLTGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIIMCSYLPIFKSFNRTKGGLIQLNHGRPQPLQYVVNAAFLASLYGDYLEAADTPGWYCGPHFYPIETLRNFARTQIEYILGKNPLKMSYVVGYGNRYPKRVHHRGASIPKNGVHYGCKGGWKWRETKKPNPNIIVGAMVAGPDRHDGFKDVRKNYNYTEATLAGNAGLVAALVALSGEGHGVDKNTMFSAVPPMFPSPPPPPAPWKP |
| Enzyme Length | 619 |
| Uniprot Accession Number | P0C1U4 |
| Absorption | |
| Active Site | ACT_SITE 167; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10140; ACT_SITE 514; /evidence=ECO:0000255|PROSITE-ProRule:PRU10059; ACT_SITE 562; /evidence=ECO:0000255|PROSITE-ProRule:PRU10060; ACT_SITE 571; /evidence=ECO:0000255|PROSITE-ProRule:PRU10060 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; |
| DNA Binding | |
| EC Number | 3.2.1.4 |
| Enzyme Function | FUNCTION: Involved in cell wall assembly during cell elongation. {ECO:0000269|PubMed:16463105}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (4); Chain (1); Glycosylation (6); Sequence conflict (1); Topological domain (2); Transmembrane (1) |
| Keywords | Carbohydrate metabolism;Cell wall biogenesis/degradation;Cellulose degradation;Glycoprotein;Glycosidase;Hydrolase;Membrane;Polysaccharide degradation;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | INDUCTION: By gibberellin (GA3) and brassinosteroid. {ECO:0000269|PubMed:16463105}. |
| Subcellular Location | SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type II membrane protein {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 24130448; 24597823; |
| Motif | |
| Gene Encoded By | |
| Mass | 68,369 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |