| IED ID | IndEnz0008000395 |
| Enzyme Type ID | cellulase000395 |
| Protein Name |
Anti-sigma-I factor RsgI6 Endo-1,4-beta-xylanase EC 3.2.1.8 |
| Gene Name | rsgI6 Cthe_2119 |
| Organism | Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Clostridium thermocellum) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Clostridia Eubacteriales Oscillospiraceae Acetivibrio Acetivibrio thermocellus (Hungateiclostridium thermocellum) (Clostridium thermocellum) Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Clostridium thermocellum) |
| Enzyme Sequence | MIVGKVLDMDEKTAIIMTDDFAFLNVVRTSEMAVGKKVKVLDSDIIKPKNSLRRYLPVAAVAACFVIVLSFVLMFINGNTARKNIYAYVGIDINPSIELWINYNNKIAEAKALNGDAETVLEGLELKEKTVAEAVNEIVQKSMELGFISREKENIILISTACDLKAGEGSENKDVQNKIGQLFDDVNKAVSDLKNSGITTRILNLTLEERESSKEENISMGRYAVYLKAKEQNVNLTIDEIKDADLLELIAKVGIDNENVPEDIVTEDKDNLDAINTGPAESAVPEVTETLPATSTPGRTEGNTATGSVDSTPALSKNETPGKTETPGRTFNTPAKSSLGQSSTPKPVSPVQTATATKGIGTLTPRNSPTPVIPSTGIQWIDQANERINEIRKRNVQIKVVDSSNKPIENAYVEAVLTNHAFGFGTAITRRAMYDSNYTKFIKDHFNWAVFENESKWYTNEPSMGIITYDDADYLYEFCRSNGIKVRGHCIFWEAEEWQPAWVRSLDPFTLRFAVDNRLNSAVGHFKGKFEHWDVNNEMIHGNFFKSRLGESIWPYMFNRAREIDPNAKYFVNNNITTLKEADDCVALVNWLRSQGVRVDGVGVHGHFGDSVDRNLLKGILDKLSVLNLPIWITEYDSVTPDEYRRADNLENLYRTAFSHPSVEGIVMWGFWERVHWRGRDASIVNDNWTLNEAGRRFESLMNEWTTRAYGSTDGSGSFGFRGFYGTYRITVTVPGKGKYNYTLNLNRGSGTLQTTYRIP |
| Enzyme Length | 760 |
| Uniprot Accession Number | A3DH97 |
| Absorption | |
| Active Site | ACT_SITE 538; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01096; ACT_SITE 635; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU01096 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.; EC=3.2.1.8; Evidence={ECO:0000269|PubMed:20820855}; |
| DNA Binding | |
| EC Number | 3.2.1.8 |
| Enzyme Function | FUNCTION: Anti-sigma factor for SigI6. Negatively regulates SigI6 activity through direct interaction. Binding of the polysaccharide substrate to the extracellular C-terminal sensing domain of RsgI6 may induce a conformational change in its N-terminal cytoplasmic region, leading to the release and activation of SigI6 (By similarity). Binds to and hydrolyzes insoluble and soluble xylan substrates. Has low enzymatic activity (PubMed:20820855). {ECO:0000250|UniProtKB:A3DBH1, ECO:0000269|PubMed:20820855}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Glycan degradation; xylan degradation. {ECO:0000255|PROSITE-ProRule:PRU01096}. |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (1); Domain (2); Region (1); Topological domain (2); Transmembrane (1) |
| Keywords | Carbohydrate metabolism;Cell membrane;Glycosidase;Hydrolase;Membrane;Polysaccharide degradation;Reference proteome;Transmembrane;Transmembrane helix;Xylan degradation |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane protein {ECO:0000255}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 85,123 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |