| IED ID | IndEnz0008000397 |
| Enzyme Type ID | cellulase000397 |
| Protein Name |
Secondary metabolism regulator LAE1 Methyltransferase LAE1 EC 2.1.1.- Velvet complex subunit LAE1 |
| Gene Name | LAE1 |
| Organism | Hypocrea atroviridis (Trichoderma atroviride) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Hypocreomycetidae Hypocreales Hypocreaceae Trichoderma Hypocrea atroviridis (Trichoderma atroviride) |
| Enzyme Sequence | MSSRNAPSGCVAPSPATAAPPSPTNLRLTVGQSGSESANEPGGEPEERILQDGFWEYGRFYGNWKKGKYNFPIDKEETSRLDILHKYFIVETEDRVTSVPLDKEGSPKIMDLGTGTGIWAFHVVEGYIPNAQIMAVDLNQIQPALIPRGVTTKQFDLEEPSWEPLLRDCDLIHLRLLYGSIRDDLWADTYRKIFEHLAPGGYVEHLEIDWTPQWDGEDHPTHSAIREWSQQFHRAMHRYRRSVKVSSEDTKRMMEAAGFTEFKETKIRCYLNPWSTDRHQREAARWFNLALGLGLEAMSLMPMIDMLHMKQEDVVDLCKRVKAETCVLRYHAYFTLHTWTAKKPASPPQ |
| Enzyme Length | 349 |
| Uniprot Accession Number | T1T504 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=L-methionyl-[protein] + S-adenosyl-L-methionine = S-adenosyl-L-homocysteine + S-methyl-L-methionyl-[protein]; Xref=Rhea:RHEA:60560, Rhea:RHEA-COMP:12313, Rhea:RHEA-COMP:15592, ChEBI:CHEBI:16044, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:142742; Evidence={ECO:0000250|UniProtKB:C8VQG9};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:60561; Evidence={ECO:0000250|UniProtKB:C8VQG9}; |
| DNA Binding | |
| EC Number | 2.1.1.- |
| Enzyme Function | FUNCTION: Methyltransferase that performs automethylation (By similarity). No other methyl-accepting substrate has been identified yet (By similarity). Component of the velvet transcription factor complex that acts as a global regulator for secondary metabolite gene expression (PubMed:23826217). Controls the expression of the gamma-pentyl-pyrone gene clusters (PubMed:23826217). Required for the expression of cellulase (PubMed:23826217). Regulates asexual sporulation (conidiation) by environmental stimuli such as light and/or mechanical injury (PubMed:23826217). Required for oxidative stress tolerance (PubMed:23826217). Plays also a role in defense and parasitism on other fungi (PubMed:23826217). {ECO:0000250|UniProtKB:C8VQG9, ECO:0000269|PubMed:23826217}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Compositional bias (1); Region (1) |
| Keywords | Methyltransferase;Nucleus;S-adenosyl-L-methionine;Sporulation;Transcription;Transcription regulation;Transferase;Virulence |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:C8VQG9}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 40,063 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:60560; RHEA:60561 |
| Cross Reference Brenda |