| IED ID | IndEnz0008000398 |
| Enzyme Type ID | cellulase000398 |
| Protein Name |
Mannan endo-1,4-beta-mannosidase 4 EC 3.2.1.78 Beta-mannanase 4 Endo-beta-1,4-mannanase 4 OsMAN4 |
| Gene Name | MAN4 Os03g0828500 LOC_Os03g61280 OSJNBa0010E04.2 |
| Organism | Oryza sativa subsp. japonica (Rice) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. japonica (Rice) |
| Enzyme Sequence | MSSCALVQRPPPWRAAVAPGDGMVAVDGTQFVVDCGRTIFFSGFNAYWLMMMAADPALRGAVATAFQQASAHGLNLARTWAFSDGGDQPLQSSPGVYNETMFQGLDFVIAEARRHGIYLLLCLTNNFDNFGGKRQYVRWAGDAGHNLTSDDDFFTSTIVKSYFKNHVKTVLTRVNTLTGVAYKDDPTIFAWELMNEPRCYADPTGAMVQAWVEEMAPYVKSVDGRHLVTPGLEGFYGAGEHESKELNPWGIYYGTNYVATHRTAAVDFATIHLYPDVWLWGSSADEQATFFRNWTRSHIDATAAYLGMPLLVTEYGKFLWKEVGANKAQRNYFLDLVLDAIYASASRGGPLVGGAFWQLLLDGDIVAGMDSLRDGYEIILAEDSRAASIIGEHSEQLAALNGQDADVLCRRASSHRRTRLGNSLSCGGGDTLELLLRMVLACFVSLSRSISSFIVQNFILL |
| Enzyme Length | 461 |
| Uniprot Accession Number | Q10B67 |
| Absorption | |
| Active Site | ACT_SITE 196; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:Q99036; ACT_SITE 314; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q99036 |
| Activity Regulation | |
| Binding Site | BINDING 80; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 195; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 274; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 357; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 364; /note=Substrate; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:B4XC07 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.; EC=3.2.1.78; |
| DNA Binding | |
| EC Number | 3.2.1.78 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Alternative sequence (1); Binding site (5); Chain (1); Sequence conflict (1) |
| Keywords | Alternative splicing;Glycosidase;Hydrolase;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 50,739 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |