| IED ID | IndEnz0008000412 |
| Enzyme Type ID | cellulase000412 |
| Protein Name |
Endo-beta-1,6-galactanase EC 3.2.1.164 |
| Gene Name | 6GAL |
| Organism | Hypocrea rufa (Trichoderma viride) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Hypocreomycetidae Hypocreales Hypocreaceae Trichoderma Hypocrea rufa (Trichoderma viride) |
| Enzyme Sequence | MRSIVLPSLALALFSQRARADTTLTIDPTSNWGTWEGWGVSLAWWAKAFGNRDDLASVFFSRNNQAVNGQTLPGLGFNIVRYNAGACSNNSYDGSTMVVSPNIKPSRQMDGFWLDWASSDPSSSSWNWNVDANQRAMLQKAKANGANIFELFSNSPMWWMCNNHNPSGSGSSDNLQSWNYQNHAVYLADIAQHAQQSWRIQFQSVEAFNEPSSSWWTAEGTQEGCHFDVSTMATVIGYLNTELSSRGLSSFVASSDENTYDLAISTWQGFNSSTRNIVKRINVHGYQDGGGRRDTLYSLASQAGKRLWNSEYGDSDASGKSMYQNLLLDFTWLHPTAWVYWQAIDGAGWGLIVGDNDNLTLSSASTKYFVLAQLTRHIRQGMQILTTPDVNTAVAYDAGSQKLVIVTANWGSAQTITFDLTRARTAGSNGATVPRWSTQTGGGDQYRSYTDTKINNGKFSASFSSGQVQTFEVSGVVLQ |
| Enzyme Length | 479 |
| Uniprot Accession Number | Q76FP5 |
| Absorption | |
| Active Site | ACT_SITE 210; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:Q45070; ACT_SITE 311; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q45070 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->6)-beta-D-galactosidic linkages in arabinogalactan proteins and (1->3):(1->6)-beta-galactans to yield galactose and beta-(1->6)-galactaobiose as the final products.; EC=3.2.1.164; Evidence={ECO:0000269|PubMed:12543554, ECO:0000269|PubMed:14565843}; |
| DNA Binding | |
| EC Number | 3.2.1.164 |
| Enzyme Function | FUNCTION: Hydrolyzes galactooligomers with a degree of polymerization higher than 3. Hydrolyzes radish root arabinogalactan-protein. Does not hydrolyze dextran, arabinan, starch, laminarin, beta-1,4- and beta-1,3-galactans, larch wood arabinogalactan or acid-insoluble polygalacturonic acid. {ECO:0000269|PubMed:12543554, ECO:0000269|PubMed:14565843}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Loses 30% of activity after 10 minutes incubation at 50 degrees Celsius, activity is abolished after 10 minutes incubation at 60 degrees Celsius. {ECO:0000269|PubMed:12543554}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 4.3. Stable between pH 3.0 and 11.0 in the presence of BSA, and pH 4.0 and 9.5 in the absence of BSA. {ECO:0000269|PubMed:12543554}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Glycosylation (3); Sequence conflict (1); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Direct protein sequencing;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..20; /evidence="ECO:0000269|PubMed:12543554, ECO:0000269|PubMed:14565843" |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 52,762 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.2.1.164; |