| IED ID | IndEnz0008000431 |
| Enzyme Type ID | cellulase000431 |
| Protein Name |
Endo-1,4-beta-xylanase F3 Xylanase F3 EC 3.2.1.8 1,4-beta-D-xylan xylanohydrolase F3 |
| Gene Name | xynF3 xlnF3 AO090001000208 |
| Organism | Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus oryzae (Yellow koji mold) Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) |
| Enzyme Sequence | MVHLKSLAGILLYTSLCIASSQQAPASINNAFVAKGKKYFGTCADQGTLSDGTNSGIIKADFGQLTPENSMKWDATEPSQGKFSFSGADYLVNYAATNNKLIRGHTLVWHSQLPSWVQGITDKNTLTSVLKNHITTVMNRYKGKVYAWDVVNEIFNEDGTLRSSVFYNVLGEDFVRIAFETARAADPQAKLYINDYNLDSANYGKTTGLANHVKKWIAQGIPIDGIGSQTHLSAGGSSGVKGALNTLAASGVSEVAITELDIAGASSNDYVNVVEACLEVSKCVGITVWGVSDKNSWRSAESPLLFDGNYQPKSAYNAILNAL |
| Enzyme Length | 323 |
| Uniprot Accession Number | Q96VB6 |
| Absorption | |
| Active Site | ACT_SITE 153; /note=Proton donor; /evidence=ECO:0000250; ACT_SITE 259; /note=Nucleophile; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.; EC=3.2.1.8; |
| DNA Binding | |
| EC Number | 3.2.1.8 |
| Enzyme Function | FUNCTION: Endo-1,4-beta-xylanase involved in the hydrolysis of xylan, a major structural heterogeneous polysaccharide found in plant biomass representing the second most abundant polysaccharide in the biosphere, after cellulose. {ECO:0000269|PubMed:11999400}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 58 degrees Celsius. The purified enzyme showed complete stability under 60 degrees Celsius. {ECO:0000269|PubMed:11999400}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 5.5. Retain 85 percent of its activity in the pH range from 6.0 to 7.0 after a 12 h incubation at 25 degrees Celsius. {ECO:0000269|PubMed:11999400}; |
| Pathway | PATHWAY: Glycan degradation; xylan degradation. |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Disulfide bond (1); Domain (1); Erroneous gene model prediction (1); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Disulfide bond;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Secreted;Signal;Xylan degradation |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:11999400, ECO:0000269|PubMed:16672490}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..23; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 34,696 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=200 uM for birch wood xylan {ECO:0000269|PubMed:11999400}; Vmax=435 umol/min/mg enzyme {ECO:0000269|PubMed:11999400}; |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |