Detail Information for IndEnz0008000438
IED ID IndEnz0008000438
Enzyme Type ID cellulase000438
Protein Name Endo-1,4-beta-xylanase 1
Xylanase 1
EC 3.2.1.8
1,4-beta-D-xylan xylanohydrolase 1
Xylanase 22
Gene Name XYL1 XYN22
Organism Magnaporthe grisea (Crabgrass-specific blast fungus) (Pyricularia grisea)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Sordariomycetidae Magnaporthales Pyriculariaceae Pyricularia Magnaporthe grisea (Crabgrass-specific blast fungus) (Pyricularia grisea)
Enzyme Sequence MVSFTSIVTAVVALAGSALAIPAPDGNMTGFPFEQLMRRQSTPSSTGRHNGYYYSWWTDGASPVQYQNGNGGSYSVQWQSGGNFVGGKGWMPGGSKSITYSGTFNPVNNGNAYLCIYGWTQNPLVEYYILENYGEYNPGNSAQSRGTLQAAGGTYTLHESTRVNQPSIEGTRTFQQYWAIRQQKRNSGTVNTGEFFQAWERAGMRMGNHNYMIVATEGYRSAGNSNINVQTPA
Enzyme Length 233
Uniprot Accession Number P0CT48
Absorption
Active Site ACT_SITE 126; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10062; ACT_SITE 217; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10063
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.; EC=3.2.1.8; Evidence={ECO:0000269|PubMed:8589407};
DNA Binding
EC Number 3.2.1.8
Enzyme Function FUNCTION: Endo-1,4-beta-xylanase involved in the hydrolysis of xylan, a major structural heterogeneous polysaccharide found in plant biomass representing the second most abundant polysaccharide in the biosphere, after cellulose. Accounts for approximately 70 percent of the endoxylanase activity in the culture filtrate. {ECO:0000269|Ref.2}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Glycan degradation; xylan degradation.
nucleotide Binding
Features Active site (2); Chain (1); Domain (1); Glycosylation (1); Signal peptide (1)
Keywords Carbohydrate metabolism;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Secreted;Signal;Xylan degradation
Interact With
Induction INDUCTION: Expressed in presence of rice cell walls or on oat spelt xylan, but not when grown on sucrose. {ECO:0000269|PubMed:8589407}.
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:8589407}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..20; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 25,491
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda