| IED ID | IndEnz0008000481 |
| Enzyme Type ID | cellulase000481 |
| Protein Name |
Endo-1,4-beta-xylanase B Xylanase B EC 3.2.1.8 1,4-beta-D-xylan xylanohydrolase B |
| Gene Name | xynB |
| Organism | Penicillium oxalicum |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Penicillium Penicillium oxalicum |
| Enzyme Sequence | MISLSSVAIALTTVVGALALPSDQSVNLAARQAITSSQTGTNNGYYYSFWTNGAGSVSYSNGAAGQFSVNWANQGGGDFTCGKGWNPGKAQDISFSGTFTPNGNAYLSIYGWTTGPLVEYYILENFGSYNPGNGMTHVGTLTSDGSDYDIYKHTQVNQPSIVGTSTFDQYWSIRKNKRSSGTVTTANHFSAWASHGMNLGSHNYQILSVEGYQSSGSASMTVSAGSSSSGGSGSGSGSGSGSGSGSGSQTTTAGSSTGTGTGSGSGSGSGGSGGNCAAQWGQCGGQGWNGPTCCSSGTCKASNQWYSQCL |
| Enzyme Length | 310 |
| Uniprot Accession Number | E7EF85 |
| Absorption | |
| Active Site | ACT_SITE 119; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10062; ACT_SITE 210; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10063 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.; EC=3.2.1.8; Evidence={ECO:0000269|PubMed:22940308, ECO:0000269|PubMed:23412069}; |
| DNA Binding | |
| EC Number | 3.2.1.8 |
| Enzyme Function | FUNCTION: Endo-1,4-beta-xylanase involved in the hydrolysis of xylan, a major structural heterogeneous polysaccharide found in plant biomass representing the second most abundant polysaccharide in the biosphere, after cellulose. Hydrolyzes birchwood xylan, beechwood xylan, and oat spelt xylan to produce short-chain xylooligosaccharides, xylopentaose, xylotriose, and xylobiose as the main products. {ECO:0000269|PubMed:22940308, ECO:0000269|PubMed:23412069}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 50 degrees Celsius. {ECO:0000269|PubMed:22940308, ECO:0000269|PubMed:23412069}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 5.0. {ECO:0000269|PubMed:22940308, ECO:0000269|PubMed:23412069}; |
| Pathway | PATHWAY: Glycan degradation; xylan degradation. |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Domain (2); Region (1); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Glycosidase;Hydrolase;Polysaccharide degradation;Secreted;Signal;Xylan degradation |
| Interact With | |
| Induction | INDUCTION: Induced by soluble xylo-oligosaccharides, with beechwood xylan being the best inducer. {ECO:0000269|PubMed:22940308}. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:23412069}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 31,282 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=5 mg/ml for birchwood xylan {ECO:0000269|PubMed:22940308, ECO:0000269|PubMed:23412069}; KM=4.6 mg/ml for beechwood xylan {ECO:0000269|PubMed:22940308, ECO:0000269|PubMed:23412069}; KM=11.3 mg/ml for oat spelt xylan {ECO:0000269|PubMed:22940308, ECO:0000269|PubMed:23412069}; Vmax=2 umol/min/mg enzyme toward birchwood xylan {ECO:0000269|PubMed:22940308, ECO:0000269|PubMed:23412069}; Vmax=2 umol/min/mg enzyme toward beechwood xylan {ECO:0000269|PubMed:22940308, ECO:0000269|PubMed:23412069}; Vmax=2.5 umol/min/mg enzyme toward oat spelt xylan {ECO:0000269|PubMed:22940308, ECO:0000269|PubMed:23412069}; |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.2.1.8; |