| IED ID | IndEnz0008000503 |
| Enzyme Type ID | cellulase000503 |
| Protein Name |
Non-reducing polyketide synthase sor2 NR-PKS sor2 EC 2.3.1.- Sorbicillinoid biosynthetic cluster protein 2 |
| Gene Name | sor2 TRIREDRAFT_73621 |
| Organism | Hypocrea jecorina (strain QM6a) (Trichoderma reesei) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Hypocreomycetidae Hypocreales Hypocreaceae Trichoderma Hypocrea jecorina (Trichoderma reesei) Hypocrea jecorina (strain QM6a) (Trichoderma reesei) |
| Enzyme Sequence | MAASSTRTLLMFGPGAMSLNETYFASILSFISTDSASQWALSAVRDIESHWPSLCEAIPKLQHTSGVSNAQKLAEWLRTGTLAPGSTIASLPNAILGPLVVIAQLVEYLRHVDSLSESGLRGGEGFQVPSAPDAETVGCCLGTFSALVVSSSSSWAQFCHNASAVVRIVFVLGALSDAQDATDASGPSVSLIAFWRGGQSLSDLKKALEKFPEAYISVLYDENRVTVTTSTRTVAALKNHLQTVGITTNETEFHGRFHAAQLYQTELEAFLAYCRRFPTFQLPDSSCIVVPTRVNSENVVTSQESLLEISCRAFLVSQFDWIKTFRAAVSSTLQNRASRIIEFGPERCVPPTLLRRLNSQVTHFDFEESIKRAKASLSHDQELPAGVAENDIAVIGMACKVAGADDVNEYWELLLQGKSQHRELIPNDRFVMETPFRPFEAGDDKKKWFGNFIGDHDAFDYKFFKKSPREALHMDPQQRLMLQAAYQAVAQSGYYNANLNVHGPTKVGCYIGVVANDYENNISHTTPIAFSATGALRSYIAGKVSHFFGWTGPAMTVDTACSASTVALDLACKAILSGECSAALVGGTNFFSTPMFFQNLAAGSFLSTTGQCKPFDAKADGYCRGEAVGTVFLKKLSQAIADGDQVLGVISATAINQNRNETPIFVPNSPSLTNVFRTAIEKSGLDAKDISVVEAHGTGTPVGDPVEYDSIRQVFGGAVRAGQDALQVGSVKGLVGHTEGASGVVALVKILLMLQRGQIPPQASFETINPSIKYSPSDNLEITKTPLPWNQEFKAALINNYGAAGSNASVIIKQGPAQLLRRLPPVVDSETEALLSKVGADDAQKAPFFISGLDEKAILAYAQKLRQFIYSHSNLDIQDLAFSVNRQSNWSLGRGAVFSAGSIAELDEKLASIETFPVPSSQPPVILCFGGQVSTFVGLDYQLFAKSAILRRHLDQCDAACKSIGAGSIYPRIFQSDPIDDPSVLQPLLFSLQYSCAMSWIDCGIVPAKLVGHSFGELTALCISGVVSLQDGLKLVYGRSKIIKESWGAERGAMLAVEADLEELESLLTTVNSSLQEGRATIACFNGPRSFTVAGSSAAIDAVQQAISNTQPVLKHKRLNVTNAFHSVLVERLKPDLEALGRQLTFALPQIPLERATRSREDHGLSPSYVANHMREPVYFHHAVERIAKEYPEAIWLEAGSNSTITTMASKALGLPKASTFQPVNVTNNSKASSQLSDVTMNLWKAGLRITFWPHSRAQTYEYKHIILPPYQFEKHRQWLEFKPPQALQVVVQTDSSTDARNGTTEPQPVGLYTLLDRGQDKYRFRVNTAAGQFVDAMSDHAIGKAQTLPAMFGVDLAIEALLSIHPELGDTSRFDPQIYNVVNQEYIHDSSRALFVLFERLGQDENSWAFELTSKGKDGAESLHMSGQLHFQASDDARSRLEFSRLDRFITHDRCLQVLESSGRSDEVIQGQTIYSVLSSSDVNYGQRLRGLQRLVGRSNESAGRLVRRRSGKLFVDFALGEVFTQVGSIWANCMAQHQRNTRNKGIYMATGLEQWSKSPKVLQKFNQGLYDNDPEIEWHVLAQHKRNTSDDSFTTDIFVFDAASGDLEEVILGIKYAPVSLDQLFSGSAIATATTPVANGYVPLTTPFVPVPTTTKQAAVPQPQHVAKKAAPRAAPKRDIKEELWLRLRPVLADISGLEPEEIQPTDALADIGIDSLMGMEMAREVETTFNCTVEQSELLSIVDVPGILKFLQSTLGDEDVHDSSETMSTVSSDGNVHSPPTSGSEMASPNLKVSYGSAYGSSDLPISAVLEAFGESSAKTDQFLKTYGCAGYLDGVSQKQTRLCLVLTSAAFKQLGCDLEAAKPGEVLQPVPFVERHRRFHQYLYSMLEETRIINIDGDVITRTAIPLPSQSADAILQDLMRRHADNGSSHQLAYNVGSRMADVLSGKADGPQLIFGDAKNRELVANFYGELPFNKLYFELMADFLTRLANKLKLSSSRSGTPTLKILEMGAGTGGTTKVLLPILAKLGIPLEYTFTDLSPSLVAQAKRRFKEYPFMKFAVHDIEQPPSDPELVGSQHVVIASNAVHATHSLRDSARNIHKFLRPDGFLMLLEMMRTLHWVDVVWGTLEGWWLFDDGRTHAIVNEQRWEKELLASGFKHVRWTDGKLPEVHVQRVIIALAGDGDGDVSDIPALTSPALKDEEDHGSKLDGEERKRVANAYVESTIRDFAIPSYTGPILSTAPGAGACVLVTGATGSLGSHLVAHIAGLPSVDTIYCLNRPRPGKKGQEDQSRDPLSRLTEVLASKSIQLSEAEISKLRVIETDLPLPQFGLDEIQYEQLLNNVTHIVHSAFPVNGLRSLKQNEPQFTLMRNLVDLAAGVSARRPTEFKFTFQFISSLSAVGMYPKVHGEKRVPEQQWDVDSALPNGYGEAKVICERVLLETLGQHPDRFRAMTVRLGQLSGSMKTGYWNHMEMLSFLFKSAQTLRALPDVDGDVSWLHLEDASASLADLLLRDAPTCHAVYHLDNPRPRDWKDVIPVLADALDIPSSHIVPFEEWLRRVRAYPGEDPWDNPSAKAMDFFEHKFKHMSCGGVTMATDHALEHSETLRAVQPVSDELVRKYIQAWKDSGFLR |
| Enzyme Length | 2633 |
| Uniprot Accession Number | G0R6S9 |
| Absorption | |
| Active Site | ACT_SITE 140; /note=Nucleophile; for transacylase activity; /evidence=ECO:0000250|UniProtKB:A0A0K0MCJ4; ACT_SITE 258; /note=Proton donor/acceptor; for transacylase activity; /evidence=ECO:0000250|UniProtKB:A0A0K0MCJ4; ACT_SITE 561; /evidence=ECO:0000255|PROSITE-ProRule:PRU10022 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 2.3.1.- |
| Enzyme Function | FUNCTION: Non-reducing polyketide synthase; part of the SOR gene cluster that mediates the biosynthesis of sorbicillinoids, a diverse group of yellow secondary metabolites that restrict growth of competing pathogenic fungi but not of bacteria (PubMed:29104566). Sorbicillinoids biosynthesis requires the action of two PKSs (PubMed:28809958). The SOR cluster is required for the production of trichodimerol and dihydrotrichotetronin, with sor2 being sufficient for production of trichodimerol, but not dihydrotrichotetronin in the light (PubMed:28809958). Sor1 iteratively combines three acetyl units and the growing chain is modified by the ketoacyl reductase subunit, and optional by the enoyl reductase subunit in the second cycle (By similarity). The polyketide is then handed over to the PKS sor2, which adds three more acetyl units, and two methyl groups (By similarity). Sor2 releases an aldehyde, which undergoes spontaneous cyclization resulting in the formation of sorbicillin or 2',3'-dihydrosorbicillin (By similarity). The monooxygenase sor5 oxidizes sorbicillin and 2',3'-dihydrosorbicillin to 2',3'-dihydrosorbicillinol and sorbicillinol, respectively (PubMed:29104566). The oxidoreductase sor8 further converts sorbicillinol into oxosorbicillinol (PubMed:29104566). Sorbicillinol is the building block for the other sorbicillinoids such as disorbicillinol, bisvertinolon, dihydrobisvertinolone, and dihydrotrichotetronine (PubMed:29104566, PubMed:28809958). {ECO:0000250|UniProtKB:B6HNK3, ECO:0000269|PubMed:28809958, ECO:0000269|PubMed:29104566}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Secondary metabolite biosynthesis. {ECO:0000269|PubMed:28809958}. |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Compositional bias (1); Domain (1); Modified residue (1); Region (7) |
| Keywords | Acyltransferase;Methyltransferase;Multifunctional enzyme;NADP;Phosphopantetheine;Phosphoprotein;Reference proteome;Transferase |
| Interact With | |
| Induction | INDUCTION: The promoter contains putative CRE1 binding motifs 5'-SYGGRG-3' and expression is differentially regulated in light and darkness by CRE1 (PubMed:28809958). Photoreceptors BLR1 and BLR2 negatively regulate the expression, while ENV1 exerts positive regulation (PubMed:28809958). Moreover the SOR biosynthetic genes show a mechanism of positive feedback on each other in darkness (PubMed:28809958). {ECO:0000269|PubMed:28809958}. |
| Subcellular Location | |
| Modified Residue | MOD_RES 1718; /note=O-(pantetheine 4'-phosphoryl)serine; /evidence=ECO:0000255|PROSITE-ProRule:PRU00258 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 288,715 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |