IED ID | IndEnz0008000540 |
Enzyme Type ID | cellulase000540 |
Protein Name |
Endo-1,4-beta-xylanase 2 Xylanase 2 EC 3.2.1.8 1,4-beta-D-xylan xylanohydrolase 2 |
Gene Name | xyn2 |
Organism | Rhizopus oryzae (Mucormycosis agent) (Rhizopus arrhizus var. delemar) |
Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Fungi incertae sedis Mucoromycota Mucoromycotina Mucoromycetes Mucorales (pin molds) Mucorineae Rhizopodaceae Rhizopus Rhizopus oryzae (Mucormycosis agent) (Rhizopus arrhizus var. delemar) |
Enzyme Sequence | MVSFSSLFVAACAAVTAFALPNELEKRAITSNEQGTNNGYFYSFWTNGGGSVSYNNGAAGQYSVNWKDCGSFTSGKGWATGSARNINFSGSFNPSGNAYLAVYGWTTSPLVEYYIMENYGEYNPGSSMAHKGTVTSDGSVYDIYAHQQVNQPSIVGTATFNQYWSIRRNKRSSGTVTTANHFNAWSRLGMGLGSHNYQIVNTEGYQSSGSASITVS |
Enzyme Length | 216 |
Uniprot Accession Number | W0HJ53 |
Absorption | |
Active Site | ACT_SITE 112; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10062; ACT_SITE 203; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10063 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.; EC=3.2.1.8; Evidence={ECO:0000269|PubMed:24760228}; |
DNA Binding | |
EC Number | 3.2.1.8 |
Enzyme Function | FUNCTION: Endo-1,4-beta-xylanase involved in the hydrolysis of xylan, a major structural heterogeneous polysaccharide found in plant biomass representing the second most abundant polysaccharide in the biosphere, after cellulose. {ECO:0000269|PubMed:24760228}. |
Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 70 degrees Celsius. {ECO:0000269|PubMed:24760228}; |
PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 5.0-6.0. {ECO:0000269|PubMed:24760228}; |
Pathway | PATHWAY: Glycan degradation; xylan degradation. |
nucleotide Binding | |
Features | Active site (2); Chain (1); Domain (1); Glycosylation (1); Signal peptide (1) |
Keywords | Carbohydrate metabolism;Direct protein sequencing;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Secreted;Signal;Xylan degradation |
Interact With | |
Induction | |
Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:24760228}. |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | SIGNAL 1..27; /evidence=ECO:0000269|PubMed:24760228 |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 23,261 |
Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=3.2 mg/ml for birchwood xylan {ECO:0000269|PubMed:24760228}; |
Metal Binding | |
Rhea ID | |
Cross Reference Brenda |