| IED ID | IndEnz0008000542 |
| Enzyme Type ID | cellulase000542 |
| Protein Name |
Endo-1,4-beta-xylanase A Xylanase A EC 3.2.1.8 1,4-beta-D-xylan xylanohydrolase A Endo-1,4-beta-xylanase G2 Xylanase G2 |
| Gene Name | xlnA xlnG2 xynG2 AO090120000026 |
| Organism | Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus oryzae (Yellow koji mold) Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) |
| Enzyme Sequence | MVSFSSILLACSAAIGALATPIEPLADHPNEAFNETAFNDLVGRSTPSSTGYNNGYYYSFWTDGGGDVTYTNGNGGSYSVQWSNVGNFVGGKGWNPGSSRAITYSGSFNPSGNGYLAVYGWTTDPLIEYYIVESYGTYNPGSGGTYKGQVTSDGGTYNIYTSVRTNAPSIIGTATFTQFWSVRTSKRVGGTVTTGNHFNAWAKYGLTLGTHNYQIVATEGYQSSGSSAITVY |
| Enzyme Length | 232 |
| Uniprot Accession Number | Q9HFA4 |
| Absorption | |
| Active Site | ACT_SITE 128; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10062; ACT_SITE 219; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10063 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.; EC=3.2.1.8; |
| DNA Binding | |
| EC Number | 3.2.1.8 |
| Enzyme Function | FUNCTION: Endo-1,4-beta-xylanase involved in the hydrolysis of xylan, a major structural heterogeneous polysaccharide found in plant biomass representing the second most abundant polysaccharide in the biosphere, after cellulose. {ECO:0000269|PubMed:11210150}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 58 degrees Celsius. Has complete stability at 60 degrees Celsius. {ECO:0000269|PubMed:11210150}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 6.0. Retains over 95 percent activity in the pH range from 5.0 to 7.0, and 70 percent activity in the pH range from 4.0 to 8.0. {ECO:0000269|PubMed:11210150}; |
| Pathway | PATHWAY: Glycan degradation; xylan degradation. |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Domain (1); Glycosylation (1); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Direct protein sequencing;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Secreted;Signal;Xylan degradation |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16672490}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 24,605 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=242.8 uM for birch wood xylan {ECO:0000269|PubMed:11210150}; Vmax=123 umol/min/mg enzyme {ECO:0000269|PubMed:11210150}; |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |