| IED ID | IndEnz0008000585 |
| Enzyme Type ID | cellulase000585 |
| Protein Name |
Endo-1,4-beta-xylanase B Xylanase B EC 3.2.1.8 1,4-beta-D-xylan xylanohydrolase B Xylanase 2 |
| Gene Name | XYLB XYL2 FGRRES_03624 FGSG_03624 |
| Organism | Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) (Wheat head blight fungus) (Fusarium graminearum) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Hypocreomycetidae Hypocreales Nectriaceae Fusarium Fusarium sambucinum species complex Gibberella zeae (Wheat head blight fungus) (Fusarium graminearum) Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) (Wheat head blight fungus) (Fusarium graminearum) |
| Enzyme Sequence | MVSFTYLLAAVSAVTGAVAAPNPTKVDAQPPSGLLEKRTSPTTGVNNGFYFSFWTDTPSAVTYTNGNGGQFSMNWNGNRGNHVGGKGWNPGAARTIKYSGDYRPNGNSYLAVYGWTRNPLVEYYIVENFGTYNPSSGAQKKGEINIDGSIYDIAVSTRNCAPSIEGDCKTFQQYWSVRRNKRSSGSVNTGAHFNAWAQAGLRLGSHDYQILAVEGYQSSGQATMTVSG |
| Enzyme Length | 228 |
| Uniprot Accession Number | I1RII8 |
| Absorption | |
| Active Site | ACT_SITE 122; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10062; ACT_SITE 214; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10063 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by the proteinaceous endoxylanase inhibitor I from T.aestivum (TAXI-I). {ECO:0000269|PubMed:15629130, ECO:0000269|PubMed:22313372, ECO:0000269|Ref.7}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.; EC=3.2.1.8; Evidence={ECO:0000269|PubMed:15629130, ECO:0000269|PubMed:22313372, ECO:0000269|Ref.7}; |
| DNA Binding | |
| EC Number | 3.2.1.8 |
| Enzyme Function | FUNCTION: Endo-1,4-beta-xylanase involved in the hydrolysis of xylan, a major structural heterogeneous polysaccharide found in plant biomass representing the second most abundant polysaccharide in the biosphere, after cellulose. Plays an important role in causing fusarium head blight (FHB) on cereal crops. Induces cell death and hydrogen peroxide accumulation in infected wheat leaves. {ECO:0000269|PubMed:22313372, ECO:0000269|PubMed:23337356, ECO:0000269|PubMed:24779355, ECO:0000269|Ref.7}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 40 degrees Celsius. {ECO:0000269|PubMed:15629130, ECO:0000269|PubMed:22313372, ECO:0000269|Ref.7}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7.0. {ECO:0000269|PubMed:15629130, ECO:0000269|PubMed:22313372, ECO:0000269|Ref.7}; |
| Pathway | PATHWAY: Glycan degradation; xylan degradation. |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Domain (1); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Secreted;Signal;Virulence;Xylan degradation |
| Interact With | |
| Induction | INDUCTION: Expression is under the control of transcription factor XYR1 and highly induced during wheat infection and by xylan. {ECO:0000269|PubMed:16707104, ECO:0000269|PubMed:17924109, ECO:0000269|PubMed:23337356}. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:15629130, ECO:0000269|PubMed:22313372}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 24,544 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=6.2 mg/ml for wheat flour arabinoxylan {ECO:0000269|PubMed:15629130, ECO:0000269|PubMed:22313372, ECO:0000269|Ref.7}; KM=4.3 mg/ml for soluble oat spelt xylan {ECO:0000269|PubMed:15629130, ECO:0000269|PubMed:22313372, ECO:0000269|Ref.7}; KM=11.9 mg/ml for soluble birchwood xylan {ECO:0000269|PubMed:15629130, ECO:0000269|PubMed:22313372, ECO:0000269|Ref.7}; Vmax=18.4 umol/min/mg enzyme toward xylan {ECO:0000269|PubMed:15629130, ECO:0000269|PubMed:22313372, ECO:0000269|Ref.7}; Vmax=61.7 umol/min/mg enzyme toward arabinoxylan {ECO:0000269|PubMed:15629130, ECO:0000269|PubMed:22313372, ECO:0000269|Ref.7}; |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |