Detail Information for IndEnz0009000024
IED ID IndEnz0009000024
Enzyme Type ID chitinase000024
Protein Name Chitinase CLP
OsCLP
EC 3.2.1.14
Chitinase-like protein
Gene Name CLP Os01g0937050 LOC_Os01g71080 P0504E02.9
Organism Oryza sativa subsp. japonica (Rice)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. japonica (Rice)
Enzyme Sequence MSLHLLLAVSLCVALASSLPWAAASANGNGNGKPLVAAITKDAATSLYTVPIKDGRPLVLDLAGALVWMSCAAAHPTLECHHHFCMHAHSYHPPGCPHNGYGRADVEDPFRCKCTAHPYNPFSGESATADLTRTRLSANATDGKNPLYPVSFAAVTSCAPDSLLAKLPAGAVGVAGLARTRLALQAQVARSQKVANKFALCLPSGGGGDGVAIFGGGPLFLLPPGRPDVAATLAGETPLHRNKDLPGYFISATKIAVNQEQVQLYTQEPLVVELCTRIPYTALRPDVYRAVVDAFARATAGRKRVTPPPPPAAPFELCYDSRDLGSTRLGYAVPQIDLVLEGGKNWTVFGGNSMAQVSDNTACLAVVKVKGEKGSPPPPAAIIGGFQMENNLVVFDEEKQRLGFSGLLWGRQTTCSNFNFTLAA
Enzyme Length 424
Uniprot Accession Number Q8S1V1
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14; Evidence={ECO:0000269|PubMed:23331415};
DNA Binding
EC Number 3.2.1.14
Enzyme Function FUNCTION: Chitinase that possesses antifungal activity (PubMed:23331415). Inhibits the growth of the fungal pathogen Rhizoctonia solani by degrading the fungal cell wall (PubMed:23331415). Does not possess inhibiting activity against fungal endo-1,4-beta-D-xylanases belonging to glycoside hydrolase family 10 (GH10) and family 11 (GH11) (PubMed:23331415). Involved in the regulation of plant growth by regulating the intracellular calcium ion concentration in roots (PubMed:28401568). {ECO:0000269|PubMed:23331415, ECO:0000269|PubMed:28401568}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Chain (1); Domain (1); Glycosylation (3); Signal peptide (1)
Keywords Apoplast;Carbohydrate metabolism;Chitin degradation;Glycoprotein;Glycosidase;Growth regulation;Hydrolase;Plant defense;Polysaccharide degradation;Reference proteome;Secreted;Signal
Interact With
Induction INDUCTION: Induced by infection with an incompatible race of the fungal pathogen Magnaporthe oryzae. {ECO:0000269|PubMed:23331415}.
Subcellular Location SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast {ECO:0000269|PubMed:28401568}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..18; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 44,620
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda