Detail Information for IndEnz0009000029
IED ID IndEnz0009000029
Enzyme Type ID chitinase000029
Protein Name Endochitinase WIN8
EC 3.2.1.14
Gene Name WIN8
Organism Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids fabids Malpighiales Salicaceae Saliceae Populus (poplars) Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa)
Enzyme Sequence MRFWALTVLSLLLSLLLGVSSDTAQCGSQAGNATCPNDLCCSSGGYCGLTVAYCCAGCVSQCRNCFFTESMFEQMLPNRNNDSCPGKGFYTYDAYFVATEFYPGFGMTGDDDTRKRELAAFFAQTSQETSGRSIIGEDAPFTWGYCLVNELNPNSDYCDPKTKSSYPCVADYYGRGPLQLRWNYNYGECGNYLGQNLLDEPEKVATDPVLSFEAALWFWMNPHSTGAPSCHEVITGEWSPSEADIEAGRKPGFGMLTNIITNGGECTKDGKTRQQNRIDYYLRYCDMLQVDPGDNLYCDNQETFEDNGLLKMVGTM
Enzyme Length 316
Uniprot Accession Number P16061
Absorption
Active Site ACT_SITE 128; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:P29022
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14;
DNA Binding
EC Number 3.2.1.14
Enzyme Function FUNCTION: Defense against chitin-containing fungal pathogens.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Chain (1); Disulfide bond (7); Domain (1); Signal peptide (1)
Keywords Carbohydrate metabolism;Chitin degradation;Chitin-binding;Disulfide bond;Glycosidase;Hydrolase;Plant defense;Polysaccharide degradation;Reference proteome;Signal
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..23; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 35,046
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda