| IED ID | IndEnz0009000035 |
| Enzyme Type ID | chitinase000035 |
| Protein Name |
Endochitinase CHI EC 3.2.1.14 |
| Gene Name | CHI LSC222 At2g43570 F18O19.32 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MAKPTSRNDRFALFFITLIFLILTVSKPVASQNCGCASDFCCSKYGYCGTTDEFCGEGCQAGPCRSSGGGGDPAVSLEGTVTPDFFNSILNQRGDCPGKGFYTHDTFMAAANSYPSFGASISKREIAAFFAHVAQETGFMCYIEEIDGPAKAASGEYCDTEKPEFPCAQGKGYYGRGAIQLSWNYNYGLCGKALDENLLASPEKVAQDQVLAFKTAFWFWTTNVRTSFKSGFGATIRAVNSRECSGGDSTAKAANRIKYFQDYCGKLGVAPGDNLTC |
| Enzyme Length | 277 |
| Uniprot Accession Number | O24603 |
| Absorption | |
| Active Site | ACT_SITE 136; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:P29022 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14; Evidence={ECO:0000250|UniProtKB:P29022}; |
| DNA Binding | |
| EC Number | 3.2.1.14 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Disulfide bond (4); Domain (1); Glycosylation (1); Region (1); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Chitin degradation;Chitin-binding;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Plant defense;Polysaccharide degradation;Reference proteome;Signal |
| Interact With | |
| Induction | INDUCTION: Accumulates during senescence and in response to 3-amino-1,2,4-triazole (3-AT) and silver nitrate (PubMed:12947053). Induced by viral infection (e.g. cucumber mosaic cucumovirus, oil seed rape tobamovirus, turnip vein clearing tobamovirus, potato virus X potexvirus, and turnip mosaic potyvirus) (PubMed:12535341). Induced by pathogens (PubMed:12920300). {ECO:0000269|PubMed:12535341, ECO:0000269|PubMed:12920300, ECO:0000269|PubMed:12947053}. |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..31; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 12034900; 12169696; 12172015; 12671089; 14742872; 15010611; 15047901; 15082927; 15144382; 15247402; 15282545; 15308753; 15448178; 15489280; 15720685; 15990873; 16021339; 16473969; 16489130; 16766691; 16920880; 17098848; 17142483; 17149585; 17194768; 17337630; 17526915; 17704230; 18296723; 18538804; 18539778; 18650403; 18682547; 19269997; 23738689; 23858430; 24755512; 28627464; 28724081; 28917191; |
| Motif | |
| Gene Encoded By | |
| Mass | 29,775 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |