| IED ID | IndEnz0009000038 |
| Enzyme Type ID | chitinase000038 |
| Protein Name |
Class V chitinase CHIT5 LjCHIT5 EC 3.2.1.14 |
| Gene Name | CHIT5 |
| Organism | Lotus japonicus (Lotus corniculatus var. japonicus) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids fabids Fabales Fabaceae Papilionoideae 50 kb inversion clade NPAAA clade Hologalegina robinioid clade Loteae Lotus Lotus japonicus (Lotus corniculatus var. japonicus) |
| Enzyme Sequence | MIIKLLVALIHYLHETMAVQSIITTPLLVILMSLRSYAFTEPSLHRQQPPSKGGVRAAYWPAWSDFSTSSIDTNYFTHIYYAFVQPAPESFNLEITESYKKWAPKYDGIHNIRPRVTTLLSIGGGGNNATLFSEMASSKQNRASFINSTIHVARKHEFNGLDLDWEWPGDEKDMSNLALLLKEWYKALVVEANTSRKSRLLLTSAVYFNSTISLIGNGPRSYPVRAIRKYLDWASPMCFDYNGAWANETGFNAALYDPNSNISTKYGIGSWIGSGVPAEKLVMGLPLYGRAWELKDPNDHGVGAKAVGPAVDTDGSMDYDEILVFNKDTGAKVVYDEVAVSFYSYSGTTWIGYDDGPSITKKVQFARSMGLKGYFFWAIGKDKDWTISKQASNAWGY |
| Enzyme Length | 397 |
| Uniprot Accession Number | A0A1B1J8Z2 |
| Absorption | |
| Active Site | ACT_SITE 166; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14; Evidence={ECO:0000255|PROSITE-ProRule:PRU10053, ECO:0000269|PubMed:27383628}; |
| DNA Binding | |
| EC Number | 3.2.1.14 |
| Enzyme Function | FUNCTION: Possesses chitinase activity in vitro toward glycol chitin, carboxymethyl-chitin, colloidal chitin, and the chitin oligosaccharides (N-acetylglucosamine) (GlcNAc)6 and (GlcNAc)5 (PubMed:27383628). Hydrolyzes (GlcNAc)6 into (GlcNAc)4 and (GlcNAc)2, or two (GlcNAc)3 molecules (PubMed:27383628). Has the capacity to inhibit hyphal growth of the fungus Trichoderma viride in an agar-plate bioassay (PubMed:27383628). Involved in symbiotic signaling (PubMed:30284535). Required for root hair infection threads (ITs) elongation and nodule development (PubMed:30284535). Possesses Nod factor (NF) hydrolase activity (PubMed:30284535). NFs are lipo-chitooligosaccharide signaling molecules produced by nitrogen-fixing rhizobia to initiate nodulation (symbiosis) on the roots of legumes (PubMed:30284535). Modulates NF levels and signaling to complete transition of infected nodules to functional nitrogen-fixing organs (PubMed:30284535). {ECO:0000269|PubMed:27383628, ECO:0000269|PubMed:30284535}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Glycan degradation; chitin degradation. {ECO:0000305}. |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Domain (1); Glycosylation (6); Mutagenesis (2); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Chitin degradation;Glycoprotein;Glycosidase;Hydrolase;Nodulation;Plant defense;Polysaccharide degradation;Signal |
| Interact With | |
| Induction | INDUCTION: Induced by the fungal pathogen Fusarium oxysporum. {ECO:0000269|PubMed:27383628}. |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..18; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 44,362 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |