| IED ID | IndEnz0009000041 |
| Enzyme Type ID | chitinase000041 |
| Protein Name |
Chitinase EC 3.2.1.14 |
| Gene Name | CHIA ORF126 |
| Organism | Autographa californica nuclear polyhedrosis virus (AcMNPV) |
| Taxonomic Lineage | Viruses Naldaviricetes Lefavirales Baculoviridae Alphabaculovirus Autographa californica multiple nucleopolyhedrovirus Autographa californica nuclear polyhedrosis virus (AcMNPV) |
| Enzyme Sequence | MLYKLLNVLWLVAVSNAIPGTPVIDWADRNYALVEINYEATAYENLIKPKEQVDVQVSWNVWNGDIGDIAYVLFDEQQVWKGDAESKRATIKVLVSGQFNMRVKLCNEDGCSVSDPVLVKVADTDGGHLAPLEYTWLENNKPGRREDKIVAAYFVEWGVYGRNFPVDKVPLPNLSHLLYGFIPICGGDGINDALKTIPGSFESLQRSCKGREDFKVAIHDPWAAVQKPQKGVSAWNEPYKGNFGQLMAAKLANPHLKILPSIGGWTLSDPFYFMHDVEKRNVFVDSVKEFLQVWKFFDGVDIDWEFPGGKGANPSLGDADGDAKTYILLLEELRAMLDDLEAQTGRVYELTSAISAGYDKIAVVNYAEAQKSLGKIFLMSYDFKGAWSNTDLGYQTTVYAPSWNSEELYTTHYAVDALLKQGVDPNKIIVGVAMYGRGWTGVTNYTNDNYFSGTGNGPGSGTWEDGVVDYRQIQKDLNNYVYTFDSAAQASYVFDKSKGDLISFDSVDSVLGKVKYVDRNKLGGLFAWEIDADNGDLLNAINAQFKPKDEL |
| Enzyme Length | 551 |
| Uniprot Accession Number | P41684 |
| Absorption | |
| Active Site | ACT_SITE 305; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14; |
| DNA Binding | |
| EC Number | 3.2.1.14 |
| Enzyme Function | FUNCTION: Plays a role in host liquefaction to facilitate horizontal transmission of the virus by hydrolyzing beta-chitin and by regulating the cysteine protease VCATH. Localized in the host reticulum endoplasmic via its KDEL motif, interacts with and thus prevents VCATH secretion before host cell lysis occurs. {ECO:0000269|PubMed:11062048, ECO:0000269|PubMed:21897030, ECO:0000269|PubMed:23302896}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Domain (1); Glycosylation (2); Motif (1); Region (1); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Chitin degradation;Glycoprotein;Glycosidase;Host endoplasmic reticulum;Hydrolase;Polysaccharide degradation;Reference proteome;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Host endoplasmic reticulum lumen {ECO:0000269|PubMed:23302896, ECO:0000269|PubMed:9811762}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..17; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 548..551; /note=Prevents secretion from ER; /evidence=ECO:0000255|PROSITE-ProRule:PRU10138 |
| Gene Encoded By | |
| Mass | 61,368 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |