| IED ID | IndEnz0009000047 |
| Enzyme Type ID | chitinase000047 |
| Protein Name |
Endochitinase 3 EC 3.2.1.14 |
| Gene Name | CHN14 |
| Organism | Nicotiana tabacum (Common tobacco) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae asterids lamiids Solanales Solanaceae Nicotianoideae Nicotianeae Nicotiana Nicotiana tabacum (Common tobacco) |
| Enzyme Sequence | MRLLEFTALSSLLVLFLLLAVSAEQCGKQAGGARCPSGMCCSNFGWCGNTQDYCGPGKCQSQCPSGPGPTPRPPTPTPGPSTGDISNIISSSMFDQMLKHRNDNTCQGKSFYTYNAFITAARSFRGFGTTGDTTRRKREVAAFFAQTSHETTGGWDTAPDGRYAWGYCYLREQGNPPSYCVQSSQWPCAPGQKYYGRGPIQISYNYNYGPCGRAIGQNLLNNPDLVATNAVVSFKSAIWFWMTAQSPKPSCHDVITGRWTPSAADRAANRLPGYGVITNIINGGLECGHGSDARVQDRIGFYRRYCSILGVSPGDNIDCGNQKSFNSGLLLETM |
| Enzyme Length | 334 |
| Uniprot Accession Number | P29059 |
| Absorption | |
| Active Site | ACT_SITE 150; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:P29022 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14; |
| DNA Binding | |
| EC Number | 3.2.1.14 |
| Enzyme Function | FUNCTION: Defense against chitin-containing fungal pathogens. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Compositional bias (1); Disulfide bond (7); Domain (1); Modified residue (3); Propeptide (1); Region (1); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Chitin degradation;Chitin-binding;Disulfide bond;Glycosidase;Hydrolase;Hydroxylation;Plant defense;Polysaccharide degradation;Reference proteome;Signal;Vacuole |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Vacuole {ECO:0000305}. Note=Vacuolar and protoplast. {ECO:0000305}. |
| Modified Residue | MOD_RES 73; /note=4-hydroxyproline; /evidence=ECO:0000250; MOD_RES 74; /note=4-hydroxyproline; /evidence=ECO:0000250; MOD_RES 76; /note=4-hydroxyproline; /evidence=ECO:0000250 |
| Post Translational Modification | PTM: The 4-hydroxyproline residues are not glycosylated in this plant vacuolar protein. {ECO:0000250}. |
| Signal Peptide | SIGNAL 1..23 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 36,221 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |