| IED ID | IndEnz0009000056 |
| Enzyme Type ID | chitinase000056 |
| Protein Name |
Exochitinase 1 EC 3.2.1.14 |
| Gene Name | chi01 |
| Organism | Streptomyces olivaceoviridis (Streptomyces corchorusii) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Streptomycetales Streptomycetaceae Streptomyces Streptomyces olivaceoviridis (Streptomyces corchorusii) |
| Enzyme Sequence | MDRFRPLAVLIAAALTLSGTTALSSAARAADADLARNGGFEAGLDGWTCTAGTTVNSPVRSGSSALKATPAGSDNARCAQTVTVQPNSQYTLSGHVQGSYVYLGASGTGTTDVSTWTQSAPDWRQLTTTFRTGPSTTRVTLYTHGWYGTGAYHADDISLVGPGGGTEQPPAPPTGLRTGSVTATSVALSWSPVTGATGYAVYRDGVKVATASGTSATVTGLTPDTAYAFQVAAVNGAGESAKSATVTATTAPGTGGGSADLPPHALVGYLHASFANGSGYTRLADVPDSWDVIDLAFGEPTSVTSGDIRFDRCPATECPNVESDAEFKAAIKAKQAAGKKVLISIGGQNGQVQLTTTAARDTFVSSVSKIIDEYGLDGLDIDFEGHSLSLNADDTDFKNPKTPVIVNLIQALKTLKAKYGDDFVLTMAPETFFVQLGYQYYGTGKWGGQDPRAGAYLPVIHALRDDLTLLHVQDYNSGPIMGLDNQYHSMGGADFHIAMTDMLLTGFPVAGDAANVFPPLRADQVAIGMPATTNAGNGHVAPAEAVKTLDCLTRKTNCGSYATHGTWPALRALMTWSINWDRFGGWEFQRTFDGYFG |
| Enzyme Length | 597 |
| Uniprot Accession Number | Q05638 |
| Absorption | |
| Active Site | ACT_SITE 384; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by the pseudosugar allosamidin A. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14; |
| DNA Binding | |
| EC Number | 3.2.1.14 |
| Enzyme Function | FUNCTION: Exochitinase that generates exclusively chitobiose from chitotetraose, chitohexaose, and colloidal high-molecular mass chitin. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 55 degrees Celsius.; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7.3.; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Domain (2); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Chitin degradation;Chitin-binding;Glycosidase;Hydrolase;Polysaccharide degradation;Signal |
| Interact With | |
| Induction | INDUCTION: By chitin. |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | PTM: The N-terminus is blocked. |
| Signal Peptide | SIGNAL 1..29; /note=Or 32; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 62,314 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |