| IED ID | IndEnz0009000063 |
| Enzyme Type ID | chitinase000063 |
| Protein Name |
Alpha-agarase EC 3.2.1.158 |
| Gene Name | agaA |
| Organism | Alteromonas agarilytica |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Alteromonadales Alteromonadaceae Alteromonas/Salinimonas group Alteromonas Alteromonas agarilytica |
| Enzyme Sequence | MFKTKRSLLNSSIAISFAVLGVQAQAETLELQAESFANSGGTYSDGQPNPVTIYNVNGQGAINFVNAGDYVDYNINALGGEYDIEYFVGTGVTSGPNIEVLVDVNGTWQSQGSVAVPYGSWDDFQSLTPSHTVTLPVGTSTVRLLAVGSTWQWNLESFRLTQVSPVEPVGDADNDGVNDNQDLCPNSPSGVTVDNNGCQITGGTDPGGESFVIQMEAFDSTGSDDSRAKGVIIGERGYPQDKHTVVDSVQTTDWVDYSINFPSSANYSVSMLASGQTDHATAVLYLDGTEINEVPVHTGSQADFANFQLAGSVYIASGTHTIRVQAQSSTGEFSWLWFGDALTFTNLDSDGGNGGEATQDADNDGVLDSSDSCPNTPTGEPADVTGCSASQLDDDNDGVSNNVDQCPNTVAGTEVDADGCEVIFADADNDGIEDSQDFCPNTPAGEAVNNSGCGASQLDADNDGVTNNIDQCPNTPAGTQVDASGCETDNGGEPGDSYYHNGQGLLFGRVDGATNFLGEEGYVANPDNYDVTTDLLETDDAIRANSTEVFRGEIYDADGHIAFYEHIDDSVRLYIDGQLVLSNDSWENSSQTTDLNLTPGWHNFELRLGNADGGSGAVSGIGFGIDVDGGTNFVHPSNLSPSMFRASGQVVVDPILPPSGGIYIQLEDFDETGTVGRVASDPNDGFVKGDSNVGWVTNGDWGKYHNVFLEAGTYRAFITVSTPAGGSYGARVDIDGEPFAWGYFDSTGGWDIAAEYELYGGHLVVESTGNHTLHVEAVGGSDWQWSGDLVRLAKVSDSAVKQPRVYNPNEHIVAEIQGPATGLQYLKTPVEIPLANKVLKSDVWYTYPQNRNLVVDGDTPYADFGATGAFWGHPPEHDFYDDTVIMDWAVNVVDDFQSEGFEYTARGEFDWGYGWFTEFTTNPQPHYVQTLDGRNVRMTFMGYLSHDGYNNNWLSNHSPAFVPFMKSQVDQILKANPDKLMFDTQTNSTRSTDMRTFGGDFSPYAMENFRVWLLKKYSNAQLVSMGINDITSFDYGAYLRAQGITHTDWSNAGDTISGNIPMMEDFIYFNRDVWNQKFAEVLEYIRQQRPNIEIGASTHLFESRGYIFNENITFLSGELNLGARTSISELPTNILVHLKGAQAVDKTLAYFPYPWEFDELRIQNAPRFGRGWVAQAYAYGGLFSIPANVWVGGEVFTWSPGADNYRDIYQFVRAQANLLDGYTSYAKAGYVHAMFSSMKAGFIDGGNQVQSSVKILTEDNINFDMLVFGDAGYPVVPRQADFDKFEYIFYDGDLNYLTAEQQAVLDAQGSKVKHIGQRGTIAGLQINVSINGSVSNETVSAVSRIHETDSTAPYVVHLINRPFAGGVTPILNNVEVAIPASYFPQGVTSAKLHLPDGSSSTVAVSTNANGDTVVSVSNLEVWGILELAH |
| Enzyme Length | 1429 |
| Uniprot Accession Number | Q9LAP7 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of 1,3-alpha-L-galactosidic linkages in agarose, yielding agarotetraose as the major product.; EC=3.2.1.158; Evidence={ECO:0000269|PubMed:8513809}; |
| DNA Binding | |
| EC Number | 3.2.1.158 |
| Enzyme Function | FUNCTION: Alpha-agarase. Does not hydrolyze agarotetraose, agarohexaose, kappa-carrageenan, iota-carrageenan or lambda-carrageenan. {ECO:0000269|PubMed:8513809}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Inactive above 60 degrees Celsius. {ECO:0000269|PubMed:8513809}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7.2. Active between pH 6.0 and 9.0. {ECO:0000269|PubMed:8513809}; |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Compositional bias (1); Domain (4); Region (2); Sequence conflict (1); Signal peptide (1) |
| Keywords | Calcium;Carbohydrate metabolism;Direct protein sequencing;Glycosidase;Hydrolase;Metal-binding;Polysaccharide degradation;Repeat;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..26; /evidence=ECO:0000269|PubMed:17513582 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 154,671 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.2.1.158; |