| IED ID | IndEnz0009000066 |
| Enzyme Type ID | chitinase000066 |
| Protein Name |
Acidic endochitinase EC 3.2.1.14 |
| Gene Name | |
| Organism | Dioscorea japonica (Japanese yam) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae Dioscoreales Dioscoreaceae Dioscorea Dioscorea japonica (Japanese yam) |
| Enzyme Sequence | QNCQCDTTIYCCSQHGYCGNSYDYCGPGCQAGPCWDPCEGDGTLTVSDIVTQEFWDGIASQAAANCPGKSFYTRSNFLEAVSAYPGFGTKCTDEDRKREIAAYFAHVTHETGHLCYIEERDGHANNYCQESQQYPCNPNKEYFGRGPMQLSWNYNYIDAGKELHFDGLNDPDIVGRDPIISFKTSLWFWIRKGVQYVILDPNQGFGATIRIINGGQECDGHNTAQMMARVGYYQEYCAQLGVSPGNNLPC |
| Enzyme Length | 250 |
| Uniprot Accession Number | P80052 |
| Absorption | |
| Active Site | ACT_SITE 110; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:P29022 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14; |
| DNA Binding | |
| EC Number | 3.2.1.14 |
| Enzyme Function | FUNCTION: Defense against chitin-containing fungal pathogens. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Disulfide bond (7); Domain (1); Modified residue (1) |
| Keywords | Carbohydrate metabolism;Chitin degradation;Chitin-binding;Direct protein sequencing;Disulfide bond;Glycosidase;Hydrolase;Plant defense;Polysaccharide degradation;Pyrrolidone carboxylic acid |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | MOD_RES 1; /note=Pyrrolidone carboxylic acid; /evidence=ECO:0000269|PubMed:1623187 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 27,908 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |