Detail Information for IndEnz0009000068
IED ID IndEnz0009000068
Enzyme Type ID chitinase000068
Protein Name Endochitinase 1
EC 3.2.1.14
Gene Name CHIA1
Organism Theobroma cacao (Cacao) (Cocoa)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Malvales Malvaceae Byttnerioideae Theobroma Theobroma cacao (Cacao) (Cocoa)
Enzyme Sequence MSFRALSVFSLFLSYLILGSAEQCGRQAGGALCPGGLCCSQFGWCGNTDDYCKKENGCQSQCSGSGGDTGGLDSLITRERFDQMLLHRNDGGCPARGFYTYDAFIAAAKSFPAFATTGDDATRKREVAAFLAQTSHETTGGAGWAAPDGPYTWGYCYNRELNPADYCQWDPNYPCAPGKQYFGRGPMQLTWNYNYGQCGRAIGVDLLNNPDLLATDPTISFKSAFWFWMTPQSPKPSCHDVIIGAWSPSGSDQAAGRVPGFGLITNIINGGLECGQGWNAKVEDRIGFYKRYCDTLGVGYGNNLDCYNQRSYNNGPSVDSM
Enzyme Length 321
Uniprot Accession Number Q41596
Absorption
Active Site ACT_SITE 137; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:P29022
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14;
DNA Binding
EC Number 3.2.1.14
Enzyme Function FUNCTION: Defense against chitin-containing fungal pathogens.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Chain (1); Disulfide bond (7); Domain (1); Region (2); Signal peptide (1)
Keywords Carbohydrate metabolism;Chitin degradation;Chitin-binding;Disulfide bond;Glycosidase;Hydrolase;Plant defense;Polysaccharide degradation;Signal;Vacuole
Interact With
Induction INDUCTION: In response to infection, elicitor, ethylene, wounding.
Subcellular Location SUBCELLULAR LOCATION: Vacuole {ECO:0000250}. Note=Vacuolar and protoplast. {ECO:0000250}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..21; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 34,848
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda