| IED ID | IndEnz0009000074 |
| Enzyme Type ID | chitinase000074 |
| Protein Name |
Probable endochitinase EC 3.2.1.14 |
| Gene Name | cht-1 C04F6.3 |
| Organism | Caenorhabditis elegans |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Nematoda (roundworms) Chromadorea Rhabditida Rhabditina Rhabditomorpha Rhabditoidea Rhabditidae Peloderinae Caenorhabditis Caenorhabditis elegans |
| Enzyme Sequence | MLLGKFLLVASFILPIAYTWTGATIRNHPADVVAARNKITSRSVARSEPTNSYIRPCYFTNWAQYRQGRAKFVPEDYTPGLCTHILFAFGWMNADYTVRAYDPADLPNDWAGEGMYRRVNKLKVTDTQLKTLLSFGGWSFGTALFQGMAASSASRKVFIDSAITFVRTWGFDGIDIDWEYPSGATDMANYVALVKELKAACESEAGSTGKDRLLVTAAVAAGPATIDAGYDIPNLAPNFDFILLMSYDFFGAWASLVGFNSPLYATTELPAEWNGWNVDSSARYWNQKGMPKEKIIVGMPTYGRGWTLNNASAINPGTSGSPAKITQYVQEAGVGAYFEFCEMLANGATRYWDSQSQVPYLVQGNQWWSYDDEESFANKMAYVKREGYGGAFVWTLDFDDFNAGCSNSNGQLYPLISVIAKELGGVIIPKKGGVTTAPTTVATTVTTGRPPMTSAVTTTTAATTTTTRAATTTTASNTNVCSGKSDGFYPNSNNCGLFVLCLSSKSYSMSCPSGLQYSASLKYCTTSTASGCSVTTTRAPTTTTKSAPTVTTTTRAPTTTTPAFKCTKDGFFGVPSDCLKFIRCVNGISYNFECPNGLSFHADTMMCDRPDPSKCAK |
| Enzyme Length | 617 |
| Uniprot Accession Number | Q11174 |
| Absorption | |
| Active Site | ACT_SITE 179; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258 |
| Activity Regulation | |
| Binding Site | BINDING 180; /note=Chitooligosaccharide; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258; BINDING 394; /note=Chitooligosaccharide; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14; |
| DNA Binding | |
| EC Number | 3.2.1.14 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (17); Binding site (2); Chain (1); Disulfide bond (3); Domain (3); Glycosylation (1); Helix (19); Region (3); Sequence conflict (3); Turn (4) |
| Keywords | 3D-structure;Carbohydrate metabolism;Chitin degradation;Chitin-binding;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Repeat |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (3) |
| Cross Reference PDB | 6LDU; 6LE7; 6LE8; |
| Mapped Pubmed ID | 10778742; 11231151; 11535590; 16184190; 16854972; 21177967; 21731485; 22267497; 22560298; 23604319; 23800452; 24884423; 25487147; 29348603; 33691404; 6593563; |
| Motif | |
| Gene Encoded By | |
| Mass | 66,857 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |