| IED ID | IndEnz0009000075 |
| Enzyme Type ID | chitinase000075 |
| Protein Name |
Endochitinase EC 3.2.1.14 |
| Gene Name | |
| Organism | Manduca sexta (Tobacco hawkmoth) (Tobacco hornworm) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Amphiesmenoptera Lepidoptera (butterflies and moths) Glossata Neolepidoptera Heteroneura Ditrysia Obtectomera Bombycoidea (hawk-moths) Sphingidae (hawkmoths) Sphinginae (small-eyed sphinx moth) Sphingini Manduca Manduca sexta (Tobacco hawkmoth) (Tobacco hornworm) |
| Enzyme Sequence | MRATLATLAVLALATAVQSDSRARIVCYFSNWAVYRPGVGRYGIEDIPVEKCTHIIYSFIGVTEGNSEVLIIDPELDVDKNGFRNFTSLRSSHPSVKFMVAVGGWAEGSSKYSHMVAQKSTRMSFIRSVVSFLKKYDFDGLDLDWEYPGAADRGGSFSDKDKFLYLVQELRRAFIRVGKGWELTAAVPLANFRLMEGYHVPELCQELDAIHVMSYDLRGNWAGFADVHSPLYKRPHDQWAYEKLNVNDGLHLWEEKGCPSNKLVVGIPFYGRSFTLSAGNNNYGLGTFINKEAGGGDPAPYTNATGFWAYYEICTEVDKDDSGWTKKWDEQGKCPYAYKGTQWVGYEDPRSVEIKMNWIKQKGYLGAMTWAIDMDDFQGLCGEKNPLIKILHKHMSSYTVPPPHTENTTPTPEWARPPSTPSDPSEGDPIPTTTTAKPASTTKTTVKTTTTTTAKPPQSVIDEENDINVRPEPKPEPQPEPEVEVPPTENEVDGSEICNSDQDYIPDKKHCDKYWRCVNGEAMQFSCQHGTVFNVELNVCDWPSNATRRECQQP |
| Enzyme Length | 554 |
| Uniprot Accession Number | P36362 |
| Absorption | |
| Active Site | ACT_SITE 146; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258 |
| Activity Regulation | |
| Binding Site | BINDING 147; /note=Chitooligosaccharide; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258; BINDING 370; /note=Chitooligosaccharide; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14; |
| DNA Binding | |
| EC Number | 3.2.1.14 |
| Enzyme Function | FUNCTION: Digests chitin in the exoskeleton during the molting process. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Binding site (2); Chain (1); Compositional bias (2); Disulfide bond (2); Domain (2); Glycosylation (3); Region (4); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Chitin degradation;Chitin-binding;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 62,204 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |