| IED ID | IndEnz0009000083 |
| Enzyme Type ID | chitinase000083 |
| Protein Name |
Chitinase EC 3.2.1.14 Fragment |
| Gene Name | |
| Organism | Streptomyces violaceus (Streptomyces venezuelae) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Streptomycetales Streptomycetaceae Streptomyces Streptomyces violaceus (Streptomyces venezuelae) |
| Enzyme Sequence | EQPGGDKVNLGYFTN |
| Enzyme Length | 15 |
| Uniprot Accession Number | P84754 |
| Absorption | |
| Active Site | |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by divalent metal ions. Maximum inhibition observed with Ca(2+) while the least inhibition was observed with Fe(2+). Inhibited by high concentrations of GlcNAc. {ECO:0000269|PubMed:16972135, ECO:0000269|Ref.1}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14; Evidence={ECO:0000269|PubMed:16972135, ECO:0000269|Ref.1}; |
| DNA Binding | |
| EC Number | 3.2.1.14 |
| Enzyme Function | FUNCTION: Antifungal activity. Inhibits the mycelial growth of A.niger, A.alternata and H.sativum. {ECO:0000269|PubMed:16972135, ECO:0000269|Ref.1}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 35 degrees Celsius. {ECO:0000269|PubMed:16972135, ECO:0000269|Ref.1}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 6-8. There is a 37.7% decrease in activity at pH 5 and a 39.8% decrease in activity at pH 9. {ECO:0000269|PubMed:16972135, ECO:0000269|Ref.1}; |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Non-terminal residue (1) |
| Keywords | Antimicrobial;Carbohydrate metabolism;Chitin degradation;Chitin-binding;Direct protein sequencing;Fungicide;Glycosidase;Hydrolase;Polysaccharide degradation |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 1,639 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |