| IED ID | IndEnz0009000091 |
| Enzyme Type ID | chitinase000091 |
| Protein Name |
Probable chitinase 10 EC 3.2.1.14 Probable chitinase 1 Probable chitinase 3 |
| Gene Name | Cht10 Cht1 Cht3 CG18140 |
| Organism | Drosophila melanogaster (Fruit fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly) |
| Enzyme Sequence | MHPYVPSVLVVVVLAISVKAHIRVDKLQIHSESNYKPAFIRSAVESIPLDIDAILLLRNSTKPEFGDAFLPLRSAVESIPKLYQSNTSRFRKNPDVRSFVRDSVESLPNDEDGLEHIEIFTKYYNSVSDVAGVDLKSKKLTHVNLDPGTISDKYAAFIARGNSEDYYPKSYRAGSPHQELLKMMNIEHTDKLDRYSDLQHQQSYGALGLANRNDLKMTKKVLCYMSNWAFYRSGEAHFVPEQIDPNLCSAIIYSFASLDPDHLTIREFDSWVDLDNQYYRRVTSLGVPVLIALGGWTDSSGSKYSRLVSDNLKRRVFISSVSSFLLRHGFSGLHLDWNYPKCWQSDCSRGPVTDRPNLTKLLRELRTEFQSVDPKFQLGVAISGYKEIIKEAYDFPALSDIVDYMTVMTYDYHGAWEQKTGHVSPLYGLSSDTYPQYNTNYTMQLLLKMGARREKLVLSIPFYGQSFTLATAHQILAGPGVAASGPGDAGELTKQPGMLAYYEICQRLTKFNWISDRNLNVIFGPFAMLNDQWVGYEDPTSAQAKARYAANNNFAGVAAWTIDLDDFRNLCCNESYPLLRAINRALGRLDSEPPTQPNCKRPTLLATPVPPQMTTISSDGSGGLGQNHDHTTSLPSGQISSSPVSTTITSTFPWWSSTTKRPREPTKTTAQPTHTTILIPAGINPVVQPSNCKSGEFFADSNNCNAYYHCFFAGELQQQFCPSGLHWNNEAKGCDWPSSAQCSLKLDQHLSTSYPNPIQTSKKPETTLKPNKKPSEISTHHQVNSTSSRPQYMRPTILECTEGDYYPHRNCRKYYICVNKALVPSECGGDLHWDGIKKLCDWPENVQCVTSKKYLKIIKSSSANEEDPCKGEKRVPYPGNCSKYLFCLWNRLQASDCPPGLHYNERIGNCDWPAAAKCNPKGSESSEEAELNAMPKPPTPQTPSSHLRPTYPTEKPVPKPRDSHYKVICYFTNWAWYRKGIGRFTPDDINTELCTHVIYGFAVLDYSELVLRTHDSWADVENNFYTRVTSLKSKGIKVSLALGGWNDSQGDKYSRLVRSPMARSRFVRHALEFIEKYGFEGLDLDWEYPVCWQTECNKGSTEEKDGFTAWVQELSEAFRPRGLMLSTAVSPSRKIIDAGYDIPQLSRYFDWIAVMTYDFHGHWDKKTGHVAPLYHHPDDDFEYFNVNYSINYWMEKGAPSQKLVMGIPLYGQSFTLENTNSSGLNAKAPAPGEAGEFTRAAGFLAYYEICERVNRQGWQVVHDEFGRMGPYAYKGTQWVSYDSPDMVRKKSLLVRSLKLGGGMVWALDLDDFKNRCGNGVHPLLTEIHNVLKDPPSLMEIPGPIETTPTEYPGMEEEIHESNGEGPEVQPIEAVMQTCENEGEEHEGILDPNHVLEEENIEATEMATEFKIICYFTNWAWYRQGGGKFLPEDIDSDLCTHIIYGFAVLSRDNLTIQPHDSWADLDNKFYERIVAYRKKGAKVTVAIGGWNDSAGDKYSRLVRNPEARSRFIRNVLDFIEEYNFDGLDLDWEYPVCWQVDCKKGTAEEKIGFSALVRELFYAFQPRGLILSAAVSPNKKVIDAGYEVAELSHYFSWISVMAYDYHGQWDKKTGHVAPMYSHPEGTANFNANFSMNYWISMGADRRKLVMGIPLYGQSFSLAETTKHQLNAPTYGGGEAGEATRARGFLAYYEICLKIRHHRWNVVRDTKGRIGPFAYHGDQWVSFDDVPMIRHKSEYIKAMGLGGAMIWALDLDDFKNVCECESYPLLKAINRVLRGFGGPQPKCLLENPKSTMKPNIKPPFRPTINAPSGPNLDSPTQNVSLNVLSIKCHSKNYLAHEWDCTKYYICEHGTYVERSCPLGLQWNKTYCDWPTNVQSSLGSNQRTQEPAVHRPNPTSVITEPPIINNSYKVVCYFTSWAWYRSSQGKFVPEDIDANLCTHLIYGFAVLDSKSLTIKTHDSWTDIDNRFYERVVEYKQRGLRVMLAIGGWNDSLGSKYARLVLNSQSRRRFVASVISFLEQHGFEGLDLAWEFPVCWQVNCNRGNPTEKDGFVALVKELSEAFKENGLILSAAVSPSKMVIDAGYNVFELSPYFDWVAVMTYDFHGHWDMRTGQIAPLFHRGGDENLYLNGNFSIHYWLERGIPNDKLVMGMPMYGQTFTLADQNRRSLNDKTVGPGKAGTFTRADGFLAYYEICEKVVNDDWKVVRDEEGIFGSYAYSGNQWISYDDVTTIRRKSQFIKSLQLGGGMIWALDLDDFRGLCGCGKHPLLRTLSQELLGIPGQKAKDCI |
| Enzyme Length | 2286 |
| Uniprot Accession Number | Q9W5U2 |
| Absorption | |
| Active Site | ACT_SITE 1087; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258; ACT_SITE 1531; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258; ACT_SITE 2030; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258 |
| Activity Regulation | |
| Binding Site | BINDING 339; /note=Chitooligosaccharide; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258; BINDING 560; /note=Chitooligosaccharide; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258; BINDING 1088; /note=Chitooligosaccharide; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258; BINDING 1305; /note=Chitooligosaccharide; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258; BINDING 1532; /note=Chitooligosaccharide; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258; BINDING 1748; /note=Chitooligosaccharide; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14; |
| DNA Binding | |
| EC Number | 3.2.1.14 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Binding site (6); Chain (1); Compositional bias (1); Disulfide bond (8); Domain (8); Erroneous initiation (1); Region (12); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Chitin degradation;Chitin-binding;Disulfide bond;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Repeat;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..20; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11914129; 12537574; 12654931; 12723700; 14630943; 14734306; 15520262; 15612038; 15883367; 16033869; 17110485; 17154718; 17594485; 18208336; 18342250; 18342251; 19232407; 21611131; 22590528; 22761891; 23071443; 24077308; 26097463; 26656493; 26801178; 26838602; 27357258; 27513559; 27582081; 29237855; 29670218; 31036678; 31722958; 32129536; 32770153; 33540716; |
| Motif | |
| Gene Encoded By | |
| Mass | 258,777 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |