| IED ID | IndEnz0009000095 |
| Enzyme Type ID | chitinase000095 |
| Protein Name |
26 kDa endochitinase 2 EC 3.2.1.14 CHI-26 |
| Gene Name | |
| Organism | Hordeum vulgare (Barley) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Pooideae Triticodae Triticeae Hordeinae Hordeum Hordeum vulgare (Barley) |
| Enzyme Sequence | MRSLAVVVAVVATVAMAIGTARGSVSSIVSRAQFDRMLLHRNDGACQAKGFYTYDAFVAAAAAFPGFGTTGSADAQKREVAAFLAQTSHETTGGWATAPDGAFAWGYCFKQERGASSDYCTPSAQWPCAPGKRYYGRGPIQLSHNYNYGPAGRAIGVDLLANPDLVATDATVGFKTAIWFWMTAQPPKPSSHAVIAGQWSPSGADRAAGRVPGFGVITNIINGGIECGHGQDSRVADRIGFYKRYCDILGVGYGNNLDCYSQRPFA |
| Enzyme Length | 266 |
| Uniprot Accession Number | P23951 |
| Absorption | |
| Active Site | ACT_SITE 90; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:P29022 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14; |
| DNA Binding | |
| EC Number | 3.2.1.14 |
| Enzyme Function | FUNCTION: Defense against chitin-containing fungal pathogens. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (3); Chain (1); Disulfide bond (3); Helix (12); Natural variant (1); Sequence conflict (2); Signal peptide (1); Turn (5) |
| Keywords | 3D-structure;Carbohydrate metabolism;Chitin degradation;Direct protein sequencing;Disulfide bond;Glycosidase;Hydrolase;Plant defense;Polysaccharide degradation;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..23 |
| Structure 3D | X-ray crystallography (2) |
| Cross Reference PDB | 1CNS; 2BAA; |
| Mapped Pubmed ID | 15299702; 7739049; |
| Motif | |
| Gene Encoded By | |
| Mass | 28,156 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.2.1.14; |