| IED ID | IndEnz0009000098 |
| Enzyme Type ID | chitinase000098 |
| Protein Name |
Chitooligosaccharide deacetylase COD EC 3.5.1.105 Chitin disaccharide deacetylase Chitobiose deacetylase Chitobiose-6P deacetylase Chitotriose deacetylase Chitotriose-6P deacetylase |
| Gene Name | chbG |
| Organism | Serratia marcescens |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Yersiniaceae Serratia Serratia marcescens |
| Enzyme Sequence | MEKLLIVNADDFGLSKGQNYGVIEAYQHGVVSSTTAMVNGGGAQHAAALSRQYPGLPIGLHFVLTHGKPLGAMPSLVNEHGELGKWLWRRAEAGELSLDEIHEELQRQFARFVTLFGRPPTHIDSHHHVHMQPQIYPLVEAFAQAQGLPLRLDREEAKRRELALQTPCSTDAFDAGFYGEMISEALFLQRLARADEQGAESLEMMCHPAFLDATILQSKYCHPRLVELDVLTAPTLKAAIAERGFLLGSFQDL |
| Enzyme Length | 253 |
| Uniprot Accession Number | Q8L3B9 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=H2O + N,N'-diacetylchitobiose = acetate + N-acetyl-beta-D-glucosaminyl-(1->4)-D-glucosamine; Xref=Rhea:RHEA:27469, ChEBI:CHEBI:15377, ChEBI:CHEBI:28681, ChEBI:CHEBI:30089, ChEBI:CHEBI:59910; EC=3.5.1.105; Evidence={ECO:0000255|HAMAP-Rule:MF_01246}; CATALYTIC ACTIVITY: Reaction=diacetylchitobiose-6'-phosphate + H2O = acetate + N'-monoacetylchitobiose-6'-phosphate; Xref=Rhea:RHEA:35083, ChEBI:CHEBI:15377, ChEBI:CHEBI:30089, ChEBI:CHEBI:64883, ChEBI:CHEBI:71315; Evidence={ECO:0000255|HAMAP-Rule:MF_01246}; |
| DNA Binding | |
| EC Number | 3.5.1.105 |
| Enzyme Function | FUNCTION: Involved in the degradation of chitin. ChbG is essential for growth on the acetylated chitooligosaccharides chitobiose and chitotriose but is dispensable for growth on cellobiose and chitosan dimer, the deacetylated form of chitobiose. Deacetylation of chitobiose-6-P and chitotriose-6-P is necessary for both the activation of the chb promoter by the regulatory protein ChbR and the hydrolysis of phosphorylated beta-glucosides by the phospho-beta-glucosidase ChbF. Catalyzes the removal of only one acetyl group from chitobiose-6-P to yield monoacetylchitobiose-6-P, the inducer of ChbR and the substrate of ChbF. {ECO:0000255|HAMAP-Rule:MF_01246}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Glycan degradation; chitin degradation. {ECO:0000255|HAMAP-Rule:MF_01246}. |
| nucleotide Binding | |
| Features | Chain (1); Erroneous initiation (1); Metal binding (2) |
| Keywords | Carbohydrate metabolism;Chitin degradation;Cytoplasm;Hydrolase;Magnesium;Metal-binding;Polysaccharide degradation |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01246}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 28,019 |
| Kinetics | |
| Metal Binding | METAL 61; /note=Magnesium; via tele nitrogen; /evidence=ECO:0000255|HAMAP-Rule:MF_01246; METAL 126; /note=Magnesium; via pros nitrogen; /evidence=ECO:0000255|HAMAP-Rule:MF_01246 |
| Rhea ID | RHEA:27469; RHEA:35083 |
| Cross Reference Brenda |