| IED ID | IndEnz0009000111 |
| Enzyme Type ID | chitinase000111 |
| Protein Name |
Basic endochitinase B EC 3.2.1.14 Pathogenesis-related protein 3 AtChiB PR-3 |
| Gene Name | CHI-B PR3 At3g12500 MQC3.32 T2E22.18 T2E22_119 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MPPQKENHRTLNKMKTNLFLFLIFSLLLSLSSAEQCGRQAGGALCPNGLCCSEFGWCGNTEPYCKQPGCQSQCTPGGTPPGPTGDLSGIISSSQFDDMLKHRNDAACPARGFYTYNAFITAAKSFPGFGTTGDTATRKKEVAAFFGQTSHETTGGWATAPDGPYSWGYCFKQEQNPASDYCEPSATWPCASGKRYYGRGPMQLSWNYNYGLCGRAIGVDLLNNPDLVANDAVIAFKAAIWFWMTAQPPKPSCHAVIAGQWQPSDADRAAGRLPGYGVITNIINGGLECGRGQDGRVADRIGFYQRYCNIFGVNPGGNLDCYNQRSFVNGLLEAAI |
| Enzyme Length | 335 |
| Uniprot Accession Number | P19171 |
| Absorption | |
| Active Site | ACT_SITE 151; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:P29022 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14; |
| DNA Binding | |
| EC Number | 3.2.1.14 |
| Enzyme Function | FUNCTION: Defense against chitin-containing fungal pathogens. Seems particularly implicated in resistance to jasmonate-inducing pathogens such as A.brassicicola. In vitro antifungal activity against T.reesei, but not against A.solani, F.oxysporum, S.sclerotiorum, G.graminis and P.megasperma. {ECO:0000269|PubMed:9844023}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Disulfide bond (7); Domain (1); Erroneous initiation (2); Motif (1); Natural variant (4); Propeptide (1); Signal peptide (1) |
| Keywords | Antimicrobial;Carbohydrate metabolism;Chitin degradation;Chitin-binding;Direct protein sequencing;Disulfide bond;Fungicide;Glycosidase;Hydrolase;Hypersensitive response;Plant defense;Polysaccharide degradation;Reference proteome;Signal;Vacuole |
| Interact With | |
| Induction | INDUCTION: Ethylene induces high levels of systemic expression of basic chitinase with expression increasing with plant age. Locally and systemically induced by jasmonic acid (JA) and pathogens such as A.brassicicola and P.syringae, particularly in case of hypersensitive responses (HR). Not induced by wounding. {ECO:0000269|PubMed:10608663, ECO:0000269|PubMed:16667600, ECO:0000269|PubMed:7610160, ECO:0000269|PubMed:9844023}. |
| Subcellular Location | SUBCELLULAR LOCATION: Vacuole {ECO:0000269|PubMed:23738689}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..33 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10594097; 11161061; 11340179; 11437246; 11950980; 11971137; 15125769; 15215502; 15231736; 15749761; 16021341; 16502469; 17114278; 17151019; 17313162; 1740436; 18203731; 18467450; 18650403; 18708476; 18944225; 19269997; 19420714; 19432948; 19832945; 19843314; 21453430; 22374398; 22852809; 23448426; 24047645; 26038230; 26304848; 28627464; 28724081; 28889908; 28917191; 29719772; 31632627; 32732351; |
| Motif | MOTIF 329..335; /note=Vacuolar targeting signal; /evidence=ECO:0000255 |
| Gene Encoded By | |
| Mass | 36,184 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |