| IED ID | IndEnz0009000115 |
| Enzyme Type ID | chitinase000115 |
| Protein Name |
Acidic endochitinase EC 3.2.1.14 |
| Gene Name | CHIB1 At5g24090 MZF18.2 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MTNMTLRKHVIYFLFFISCSLSKPSDASRGGIAIYWGQNGNEGNLSATCATGRYAYVNVAFLVKFGNGQTPELNLAGHCNPAANTCTHFGSQVKDCQSRGIKVMLSLGGGIGNYSIGSREDAKVIADYLWNNFLGGKSSSRPLGDAVLDGIDFNIELGSPQHWDDLARTLSKFSHRGRKIYLTGAPQCPFPDRLMGSALNTKRFDYVWIQFYNNPPCSYSSGNTQNLFDSWNKWTTSIAAQKFFLGLPAAPEAAGSGYIPPDVLTSQILPTLKKSRKYGGVMLWSKFWDDKNGYSSSILASV |
| Enzyme Length | 302 |
| Uniprot Accession Number | P19172 |
| Absorption | |
| Active Site | ACT_SITE 156; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14; |
| DNA Binding | |
| EC Number | 3.2.1.14 |
| Enzyme Function | FUNCTION: This protein functions as a defense against chitin containing fungal pathogens. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Disulfide bond (3); Domain (1); Natural variant (18); Sequence conflict (1); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Chitin degradation;Disulfide bond;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | INDUCTION: Expression of the acidic chitinase gene was not detected in normal, untreated plants nor in plants treated with ethylene or salicylic acid. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, extracellular space. Note=Intercellular space of infected plants. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..30 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 12942173; 14535885; 15769804; 16273343; 18315867; 19420714; 22936594; 24957336; |
| Motif | |
| Gene Encoded By | |
| Mass | 33,097 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |