| IED ID | IndEnz0009000118 |
| Enzyme Type ID | chitinase000118 |
| Protein Name |
Endochitinase EC 3.2.1.14 MF1 antigen |
| Gene Name | |
| Organism | Brugia malayi (Filarial nematode worm) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Nematoda (roundworms) Chromadorea Rhabditida Spirurina Spiruromorpha Filarioidea Onchocercidae Brugia Brugia malayi (Filarial nematode worm) |
| Enzyme Sequence | MNRTTLILFFIILSNTITVIHGYVRGCYYTNWAQYRDGEGKFLPGNIPNGLCTHILYAFAKVDELGDSKPFEWNDEDTEWSKGMYSAVTKLRETNPGLKVLLSYGGYNFGSAIFTGIAKSAQKTERFIKSAIAFLRKNNFDGFDLDWEYPVGVAEEHAKLVEAMKTAFVEEAKTSGKQRLLLTAAVSAGKGTIDGSYNVESLGKNFDLLFLMSYDLHGSWEKNVDLHGKLHPTKGEVSGIGIFNTEFAADYWASKGMPKEKIIIGIPMYAQGWTLDNPSETAIGAAASRPSSASKTNPAGGTASYWEICKYLKEGGKETVHQEGVGAYMVKGDQWYGYDNEETIRIKMKWLKEKGYGGAFIWALDFDDFTGKSCGKGPYPLLNAISSELEGESENPEITTEEPSITETEAYETDETEETSETEAYDTDETEETSETEATTYDTDETEGQECPERDGLFPHPTDCHLFIQCANNIAYVMQCPATTFFNDAIKVCDHMTNAPDTCI |
| Enzyme Length | 504 |
| Uniprot Accession Number | P29030 |
| Absorption | |
| Active Site | ACT_SITE 148; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258 |
| Activity Regulation | |
| Binding Site | BINDING 149; /note=Chitooligosaccharide; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258; BINDING 362; /note=Chitooligosaccharide; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14; |
| DNA Binding | |
| EC Number | 3.2.1.14 |
| Enzyme Function | FUNCTION: Microfilarial chitinase, which may function to degrade chitin-containing structures in the micro-filaria or in its mosquito vector during parasite development and transmission. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Binding site (2); Chain (1); Compositional bias (2); Disulfide bond (2); Domain (2); Region (5); Repeat (3); Signal peptide (1) |
| Keywords | Calcium;Carbohydrate metabolism;Chitin degradation;Chitin-binding;Direct protein sequencing;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Repeat;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | PTM: O-glycosylated. |
| Signal Peptide | SIGNAL 1..22 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 55,971 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.2.1.14; |