| IED ID | IndEnz0009000125 |
| Enzyme Type ID | chitinase000125 |
| Protein Name |
Putative type II secretion system protein E T2SS protein E EC 7.4.2.8 Putative general secretion pathway protein E Type II traffic warden ATPase |
| Gene Name | gspE yheG b3326 JW3288 |
| Organism | Escherichia coli (strain K12) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Escherichia coli (strain K12) |
| Enzyme Sequence | MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQVGQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDIDQSVDLLSLSEEMPANEDLLNEDSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALLISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQLSLNNLGMTAADKQDLENLIQLPHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTVEDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEIRDTETAQIAVQASLTGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVSPQQAQMFKYHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQALERLVRQQHKALIKNGLQKVISGDTSWDEVMRVASATLESEA |
| Enzyme Length | 493 |
| Uniprot Accession Number | P45759 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=ATP + H(2)O + cellular protein(Side 1) = ADP + phosphate + cellular protein(Side 2).; EC=7.4.2.8; Evidence={ECO:0000250|UniProtKB:P37093}; |
| DNA Binding | |
| EC Number | 7.4.2.8 |
| Enzyme Function | FUNCTION: ATPase component of the type II secretion system required for the energy-dependent secretion of extracellular factors such as proteases and toxins from the periplasm. Acts as a molecular motor to provide the energy that is required for assembly of the pseudopilus and the extrusion of substrates generated in the cytoplasm. {ECO:0000250|UniProtKB:Q00512}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 249..256; /note=ATP; /evidence=ECO:0000255 |
| Features | Chain (1); Metal binding (4); Nucleotide binding (1) |
| Keywords | ATP-binding;Cell inner membrane;Cell membrane;Membrane;Metal-binding;Nucleotide-binding;Protein transport;Reference proteome;Translocase;Transport;Zinc |
| Interact With | P60422 |
| Induction | INDUCTION: Silenced by the DNA-binding protein H-NS under standard growth conditions. {ECO:0000269|PubMed:11118204}. |
| Subcellular Location | SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250|UniProtKB:Q00512}. Note=Membrane association is not an intrinsic property but requires the GspL gene product. {ECO:0000250|UniProtKB:Q00512}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 16606699; 24561554; 24627523; |
| Motif | |
| Gene Encoded By | |
| Mass | 54,611 |
| Kinetics | |
| Metal Binding | METAL 382; /note=Zinc; /evidence=ECO:0000250|UniProtKB:P37093; METAL 385; /note=Zinc; /evidence=ECO:0000250|UniProtKB:P37093; METAL 415; /note=Zinc; /evidence=ECO:0000250|UniProtKB:P37093; METAL 418; /note=Zinc; /evidence=ECO:0000250|UniProtKB:P37093 |
| Rhea ID | |
| Cross Reference Brenda |