| IED ID | IndEnz0009000139 |
| Enzyme Type ID | chitinase000139 |
| Protein Name |
Secondary metabolism regulator laeA Methyltransferase laeA EC 2.1.1.- Velvet complex subunit laeA |
| Gene Name | laeA Pc16g14010 PCH_Pc16g14010 |
| Organism | Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255) (Penicillium chrysogenum) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Penicillium Penicillium chrysogenum species complex Penicillium rubens Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255) (Penicillium chrysogenum) |
| Enzyme Sequence | MSYRESSGSFPAPDRTSLPKMFTNGDSRLRHLPPISSPPPPKRYKSESTPGSDAGHSRYYSHSVASDRTRSRQPSSAMDLYTLIDRDPVDKDPRRNARFTSNGSVATQASHASNASQISRSSPIIISDRKIPEKYPNHKENGRMYHGYRKGIYPLPCDEEEQDRLDIFHKLFTVARAEDGLIYAPHPPGSRILDLGCGTGIWSIEVANKFPGSFVVGVDLAPIQPTNTPKNCDFYAPFDFEAPWTMGEDSWDIIHMQMGCGSVASWPSLYRRVFQHLKPGGWFEQVEIDFRPRVEDKDGEPGRAMANWYSTLKHATEATMRPLAHSSNETIRNLQEAGFTEIDHQIVGLPMNPWHPDSHEQKVARWYNLAISESVQPMCLAPFSRVLSWTREQIDRIAFDVKQEAFDKRIKTYNLLHIYQARKPVEE |
| Enzyme Length | 427 |
| Uniprot Accession Number | B6H9U8 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=L-methionyl-[protein] + S-adenosyl-L-methionine = S-adenosyl-L-homocysteine + S-methyl-L-methionyl-[protein]; Xref=Rhea:RHEA:60560, Rhea:RHEA-COMP:12313, Rhea:RHEA-COMP:15592, ChEBI:CHEBI:16044, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:142742; Evidence={ECO:0000250|UniProtKB:C8VQG9};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:60561; Evidence={ECO:0000250|UniProtKB:C8VQG9}; |
| DNA Binding | |
| EC Number | 2.1.1.- |
| Enzyme Function | FUNCTION: Methyltransferase that performs automethylation (By similarity). No other methyl-accepting substrate has been identified yet (By similarity). Component of the velvet transcription factor complex that acts as a global regulator for secondary metabolite gene expression (PubMed:18952140, PubMed:22439693). Controls the expression of the penicillin gene cluster (PubMed:18952140, PubMed:20543063, PubMed:22439693, PubMed:23089625, PubMed:23264641). Controls benzylpenicillin production, but is not involved in the biosynthesis of the alkaloid roquefortine C (PubMed:18952140). Positively controls the expression of the class V chitinase chiB1 (PubMed:21816879). Positively controls the expression of the transcription factor atfA (PubMed:25557366). Regulates also asexual differentiation (PubMed:18952140, PubMed:23264641). {ECO:0000250|UniProtKB:C8VQG9, ECO:0000269|PubMed:18952140, ECO:0000269|PubMed:20543063, ECO:0000269|PubMed:21816879, ECO:0000269|PubMed:22439693, ECO:0000269|PubMed:23089625, ECO:0000269|PubMed:23264641, ECO:0000269|PubMed:25557366}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Compositional bias (2); Region (1) |
| Keywords | Methyltransferase;Nucleus;Reference proteome;S-adenosyl-L-methionine;Sporulation;Transcription;Transcription regulation;Transferase |
| Interact With | |
| Induction | INDUCTION: Expression is negatively regulated by velA (PubMed:20543063). Expression is stimulated by the polyamines 1,3-diaminopropane (1,3-DAP) and spermidine (PubMed:23089625). {ECO:0000269|PubMed:20543063, ECO:0000269|PubMed:23089625}. |
| Subcellular Location | SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:20543063}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 48,548 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:60560; RHEA:60561 |
| Cross Reference Brenda |