| IED ID | IndEnz0009000140 |
| Enzyme Type ID | chitinase000140 |
| Protein Name |
Nod factor hydrolase protein 1 MtNFH1 Class V chitinase NFH1 Mtchit5 |
| Gene Name | NFH1 CHIT5 MTR_4g116990 |
| Organism | Medicago truncatula (Barrel medic) (Medicago tribuloides) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids fabids Fabales Fabaceae Papilionoideae 50 kb inversion clade NPAAA clade Hologalegina IRL clade Trifolieae Medicago (medics) Medicago truncatula (Barrel medic) (Medicago tribuloides) |
| Enzyme Sequence | MANFLKLKQFLTLVLILLALAAKSSKSTPSPSSTTRVKGIYWIENPLFPPSSIDTSLFTHIFYAFVSPNKFTYKLEEEEEDSTTVATSLTTFTNTFKTKTPPIPTLLSIGGATSNSTLFAFIASDPTARATFINSTIQVARTFGFDGIDFDWEFPTTTKEMNDLGELLFQWRRAISDETASTSRPPLLLTAAVYFAVNFFLSGERRMYPVDSINKNLDWVNVMSYDLRGSGSNVTGAPSGMFDSKSNVSVVSGLFSWIRGGVAPEKIVMGMPLYGKSWKLQDPNVHGIGAPNVGPGPGVDGGMAYFQVVDFNKQMGAKVVYDKETGSVYSYSGSTWIGYDDPFTVSVKVGFAQALKLGGYFFWAAGYDTSDWKVSTASKAWRPES |
| Enzyme Length | 385 |
| Uniprot Accession Number | U5N4E3 |
| Absorption | |
| Active Site | ACT_SITE 153; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Symbiotic enzyme that hydrolytically inactivates Nod factors (NFs) with a C16:2 acyl chain produced by the microsymbiont Sinorhizobium meliloti (PubMed:27383628, PubMed:29367305). NFs are lipo-chitooligosaccharide signaling molecules produced by nitrogen-fixing rhizobia to initiate nodulation (symbiosis) on the roots of legumes (PubMed:27383628, PubMed:29367305). Controls NF hydrolysis at the stage of root hair infection (PubMed:29367305). Involved in the regulation of growth and branching of mature nodules (PubMed:29367305). Modulates NF levels and signaling to complete transition of infected nodules to functional nitrogen-fixing organs (PubMed:29367305). Lacks chitinase activity in vitro toward glycol chitin, carboxymethyl-chitin, colloidal chitin, and the chitin oligosaccharides (N-acetylglucosamine) (GlcNAc)6 and (GlcNAc)5 (PubMed:27383628). {ECO:0000269|PubMed:27383628, ECO:0000269|PubMed:29367305}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Domain (1); Glycosylation (4); Mutagenesis (3); Sequence conflict (14); Signal peptide (1) |
| Keywords | Glycoprotein;Glycosidase;Hydrolase;Nodulation;Reference proteome;Signal |
| Interact With | |
| Induction | INDUCTION: Induced in roots by Nod factors and infection of the microsymbiont Sinorhizobium meliloti. {ECO:0000269|PubMed:15107993, ECO:0000269|PubMed:29367305}. |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..21; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 42,193 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |