| IED ID | IndEnz0009000167 |
| Enzyme Type ID | chitinase000167 |
| Protein Name |
Killer toxin subunits alpha/beta RF2 protein Cleaved into: Killer toxin subunit alpha; Killer toxin subunit beta EC 3.2.1.14 Endochitinase |
| Gene Name | |
| Organism | Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Kluyveromyces Kluyveromyces lactis (Yeast) (Candida sphaerica) Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica) |
| Enzyme Sequence | MNIFYIFLFLLSFVQGLEHTHRRGSLVKRAVCYDTDQVPLNIFFGYNRADKTDSNKNMALNIFNVFRGFLAGEGGESFYNSNGNVYGFMWVGSMVHNRGFKDNILPIMENEVKNYGIPKTLYLEYDGGGDPMKSFGIILDTTSRDTVVKAAKLWSQGKKLNSYEGSKNYQATACYLSYAYRKPIVNDNFVGTCDYFTLESGKTPADQSGINGESLQGYNPNLDFSKLSAGQPICKTIGNPPNFKPSKNSDGSCKTYKVSSGESCSSIAVKYYPLSLNDIENYNKGNYGWKGCSSLQKDYNLCVSDGSAPRPVSNPIAECGPLAPGEKYNAKCPLNACCSEFGFCGLTKDYCDKKSSTTGAPGTDGCFSNCGYGSTSNVKSSTFKKIAYWLDAKDKLAMDPKNIPNGPYDILHYAFVNINSDFSIDDSAFSKSAFLKVTSSKKIPSFGGWDFSTSPSTYTIFRNAVKTDQNRNTFANNLINFMNKYNLDGIDLDWEYPGAPDIPDIPADDSSSGSNYLTFLKLLKGKMPSGKTLSIAIPSSYWYLKNFPISDIQNTVDYMVYMTYDIHGIWEYGKANSYINCHTPRKEIEDAIKMLDKAGVKFNKVFGGVANYGRSYKMVNTNCYNYGCGFQREGGNSRDMTNTPGVLSDSEIIDIDSSDKKNDRWVDTNTDCIFMKYDGNSVVSWPKSRYDLEDMFKNYGFAGTSLWAANYFKHDEWKNDEDDNNDDTEDPFDEENVYFDVYDCKNKAGYDLDNPVYGCRLETAINIIIWNGTESVNTVLNILNDYDNYIKYYEALTRAHYDSVMEKYEKWLFEEDGYYTYYTDVDGDDIIITPPDKKKRDYIQEKYSFEKEFMMSQNMTELTEIKVNKTINFMLNGTSLAVKEYNNEKVLYKRGDIPPPGSNNRLIRNSIILDKDKEAAIASFKQYSGIELSKDSFVQRDKDKKFDLNGKHYTFMHSTILNAIVLFPNVLTNIDSDYIHHISDLIEQAHNSLGNESPDNIYEVLESVVVFMSVSEIADYTYTEGKKIKEKYDKMKKTMIVGIILGIIGGLSLFLGPIGIATSVLADFALLGADAAINGELNPSDLAFALAGLFLPVFASLGKTFKFAEALQKININKSKNFDNLNEFEKIRFFRSKLGKVKMCGS |
| Enzyme Length | 1146 |
| Uniprot Accession Number | P09805 |
| Absorption | |
| Active Site | ACT_SITE 495; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258 |
| Activity Regulation | |
| Binding Site | BINDING 496; /note=Chitooligosaccharide; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258; BINDING 707; /note=Chitooligosaccharide; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14; |
| DNA Binding | |
| EC Number | 3.2.1.14 |
| Enzyme Function | FUNCTION: The alpha subunit is a potent exochitinase. Along with the beta subunit it plays a role in the initial interaction of the toxin with sensitive cells and allow the gamma subunit (the active toxin) to gain entry into the cell. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Binding site (2); Chain (2); Disulfide bond (4); Domain (4); Glycosylation (5); Propeptide (1); Region (3); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Chitin degradation;Chitin-binding;Cleavage on pair of basic residues;Direct protein sequencing;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Plasmid;Polysaccharide degradation;Reference proteome;Repeat;Signal;Toxin |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | PTM: RF2 is potentially split by membrane-bound basic amino acid-specific peptidase to yield the alpha and beta subunits. |
| Signal Peptide | SIGNAL 1..17; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | Plasmid pGKl-1 |
| Mass | 128,937 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |