| IED ID |
IndEnz0009000170 |
| Enzyme Type ID |
chitinase000170 |
| Protein Name |
Nitrate/nitrite binding protein NrtA
|
| Gene Name |
nrtA sll1450 |
| Organism |
Synechocystis sp. (strain PCC 6803 / Kazusa) |
| Taxonomic Lineage |
cellular organisms
Bacteria
Terrabacteria group
Cyanobacteria/Melainabacteria group
Cyanobacteria
Synechococcales
Merismopediaceae
Synechocystis
unclassified Synechocystis
Synechocystis sp. PCC 6803
Synechocystis sp. (strain PCC 6803 / Kazusa)
|
| Enzyme Sequence |
MSNFSRSTRRKFMFTAGAAAIGGVVLHGCTSPTTTSTGTGTGSSTDQAISPLVEGENAPEVTTAKLGFIALTDAAPLIIAKEKGFYAKYGMPDVEVLKQASWGTTRDNLVLGSASGGIDGAHILTPMPYLITMGTVTDGKPTPMYILARLNVNGQGIQLGNNYKDLKVGTDAAPLKEAFAKVTDPKVAMTFPGGTHDMWIRYWLAAGGMEPGKDFSTIVVPPAQMVANVKVNAMESFCVGEPWPLQTVNQGVGYQALTTGQLWKDHPEKAFGMRADWVDQNPKAAKALLMAVMEAQQWCDQAENKEEMCQILSKREWFKVPFEDIIDRSKGIYNFGNGQETFEDQEIMQKYWVDNASYPYKSHDQWFLTENIRWGYLPASTDTKAIVDKVNREDLWREAAQALEVPADQIPSSPSRGIETFFDGITFDPENPQAYLDSLKIKSIKA |
| Enzyme Length |
446 |
| Uniprot Accession Number |
P73452 |
| Absorption |
|
| Active Site |
|
| Activity Regulation |
|
| Binding Site |
BINDING 102; /note="Nitrate"; /evidence="ECO:0000269|PubMed:16777960, ECO:0007744|PDB:2G29"; BINDING 155; /note="Nitrate"; /evidence="ECO:0000269|PubMed:16777960, ECO:0007744|PDB:2G29"; BINDING 196; /note="Nitrate"; /evidence="ECO:0000269|PubMed:16777960, ECO:0007744|PDB:2G29"; BINDING 240; /note="Nitrate; via amide nitrogen"; /evidence="ECO:0000269|PubMed:16777960, ECO:0007744|PDB:2G29"; BINDING 269; /note="Nitrate"; /evidence="ECO:0000269|PubMed:16777960, ECO:0007744|PDB:2G29" |
| Calcium Binding |
|
| catalytic Activity |
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| DNA Binding |
|
| EC Number |
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| Enzyme Function |
FUNCTION: Part of the ABC transporter complex NrtABCD involved in nitrate uptake (PubMed:16777960). The complex is probably also involved in nitrite transport (By similarity). NrtA is the substrate-binding protein (PubMed:16777960). Binds nitrate (PubMed:16777960). {ECO:0000250|UniProtKB:P38043, ECO:0000269|PubMed:16777960}. |
| Temperature Dependency |
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| PH Dependency |
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| Pathway |
|
| nucleotide Binding |
|
| Features |
Beta strand (15); Binding site (5); Chain (1); Helix (25); Lipidation (2); Signal peptide (1); Turn (3) |
| Keywords |
3D-structure;Cell inner membrane;Cell membrane;Ion transport;Lipoprotein;Membrane;Nitrate assimilation;Palmitate;Reference proteome;Signal;Transport |
| Interact With |
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| Induction |
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| Subcellular Location |
SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305|PubMed:16777960}; Lipid-anchor {ECO:0000305|PubMed:16777960}; Periplasmic side {ECO:0000305|PubMed:16777960}. |
| Modified Residue |
|
| Post Translational Modification |
|
| Signal Peptide |
SIGNAL 1..28; /evidence=ECO:0000255 |
| Structure 3D |
X-ray crystallography (1) |
| Cross Reference PDB |
2G29;
|
| Mapped Pubmed ID |
- |
| Motif |
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| Gene Encoded By |
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| Mass |
48,967 |
| Kinetics |
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| Metal Binding |
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| Rhea ID |
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| Cross Reference Brenda |
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