| IED ID | IndEnz0009000172 |
| Enzyme Type ID | chitinase000172 |
| Protein Name |
Type II secretion system protein H T2SS minor pseudopilin H General secretion pathway protein H Protein transport protein HofH Putative general secretion pathway protein H |
| Gene Name | gspH hofH hopH b3329 JW3291 |
| Organism | Escherichia coli (strain K12) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Escherichia coli (strain K12) |
| Enzyme Sequence | MNQQRGFTLLEMMLVLALVAITASVVLFTYGREDVASTRARETAARFTAALELAIDRATLSGQPVGIHFSDSAWRIMVPGKTPSAWRWVPLQEDAADESQNDWDEELSIHLQPFKPDDSNQPQVVILADGQITPFSLLMANAGTGEPLLTLVCSGSWPLDQTLARDTRP |
| Enzyme Length | 169 |
| Uniprot Accession Number | P41443 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Component of the type II secretion system required for the energy-dependent secretion of extracellular factors such as proteases and toxins from the periplasm. Part of the pseudopilus tip complex that is critical for the recognition and binding of secretion substrates. {ECO:0000250|UniProtKB:Q00515}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Beta strand (11); Chain (1); Helix (1); Modified residue (1); Propeptide (1); Transmembrane (1); Turn (3) |
| Keywords | 3D-structure;Cell inner membrane;Cell membrane;Membrane;Methylation;Protein transport;Reference proteome;Transmembrane;Transmembrane helix;Transport |
| Interact With | P76086 |
| Induction | INDUCTION: Silenced by the DNA-binding protein H-NS under standard growth conditions. {ECO:0000269|PubMed:11118204}. |
| Subcellular Location | SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250|UniProtKB:Q00515}; Single-pass membrane protein {ECO:0000255}. |
| Modified Residue | MOD_RES 7; /note=N-methylphenylalanine; /evidence=ECO:0000255|PROSITE-ProRule:PRU01070 |
| Post Translational Modification | PTM: Cleaved by prepilin peptidase. {ECO:0000250|UniProtKB:Q00515}.; PTM: Methylated by prepilin peptidase at the amino group of the N-terminal phenylalanine once the leader sequence is cleaved by prepilin peptidase. {ECO:0000250|UniProtKB:Q00515}. |
| Signal Peptide | |
| Structure 3D | NMR spectroscopy (1) |
| Cross Reference PDB | 2KNQ; |
| Mapped Pubmed ID | 16606699; 24561554; |
| Motif | |
| Gene Encoded By | |
| Mass | 18,565 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |