| IED ID | IndEnz0009000187 |
| Enzyme Type ID | chitinase000187 |
| Protein Name |
Chitinase A1 EC 3.2.1.14 |
| Gene Name | chiA1 |
| Organism | Niallia circulans (Bacillus circulans) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Niallia Niallia circulans (Bacillus circulans) |
| Enzyme Sequence | MINLNKHTAFKKTAKFFLGLSLLLSVIVPSFALQPATAEAADSYKIVGYYPSWAAYGRNYNVADIDPTKVTHINYAFADICWNGIHGNPDPSGPNPVTWTCQNEKSQTINVPNGTIVLGDPWIDTGKTFAGDTWDQPIAGNINQLNKLKQTNPNLKTIISVGGWTWSNRFSDVAATAATREVFANSAVDFLRKYNFDGVDLDWEYPVSGGLDGNSKRPEDKQNYTLLLSKIREKLDAAGAVDGKKYLLTIASGASATYAANTELAKIAAIVDWINIMTYDFNGAWQKISAHNAPLNYDPAASAAGVPDANTFNVAAGAQGHLDAGVPAAKLVLGVPFYGRGWDGCAQAGNGQYQTCTGGSSVGTWEAGSFDFYDLEANYINKNGYTRYWNDTAKVPYLYNASNKRFISYDDAESVGYKTAYIKSKGLGGAMFWELSGDRNKTLQNKLKADLPTGGTVPPVDTTAPSVPGNARSTGVTANSVTLAWNASTDNVGVTGYNVYNGANLATSVTGTTATISGLTAGTSYTFTIKAKDAAGNLSAASNAVTVSTTAQPGGDTQAPTAPTNLASTAQTTSSITLSWTASTDNVGVTGYDVYNGTALATTVTGTTATISGLAADTSYTFTVKAKDAAGNVSAASNAVSVKTAAETTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNVPALWQLQ |
| Enzyme Length | 699 |
| Uniprot Accession Number | P20533 |
| Absorption | |
| Active Site | ACT_SITE 204; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258 |
| Activity Regulation | |
| Binding Site | BINDING 205; /note=Chitooligosaccharide; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258; BINDING 433; /note=Chitooligosaccharide; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14; |
| DNA Binding | |
| EC Number | 3.2.1.14 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (30); Binding site (2); Chain (1); Domain (3); Helix (19); Mutagenesis (3); Region (4); Signal peptide (1); Turn (6) |
| Keywords | 3D-structure;Carbohydrate metabolism;Chitin degradation;Glycosidase;Hydrolase;Polysaccharide degradation;Repeat;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..41 |
| Structure 3D | X-ray crystallography (1); NMR spectroscopy (2) |
| Cross Reference PDB | 1ED7; 1ITX; 1K85; |
| Mapped Pubmed ID | 10788483; 11600504; |
| Motif | |
| Gene Encoded By | |
| Mass | 73,678 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |