Detail Information for IndEnz0009000194
IED ID IndEnz0009000194
Enzyme Type ID chitinase000194
Protein Name Acidic endochitinase WIN6.2B
EC 3.2.1.14
Gene Name
Organism Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids fabids Malpighiales Salicaceae Saliceae Populus (poplars) Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa)
Enzyme Sequence MSVWAFAFFSLFLSLSVRGSAEQCGQQAGDALCPGGLCCSSYGWCGTTADYCGDGCQSQCDGGGGGGGGGGGGGGGGGGGGDGYLSDIIPESMFDDMLKYRNDPQCPAVGFYTYNAFISAAKEFPDFGNTGDDLMRKREIAAFLGQTSHETNGWWPAAQGDQYDWGYCHMNYNYGLCGDDLNLPLLQEPELVETDPFIAFKTALWFWMTPQSPKPSCHAVITESWTPSAADSEAGRVPGYGVITNIINGGIECGQGGPNNANENRIGFYKTYCDSLGTTYGSNLDCYQQRPFGYGLLGLKDTM
Enzyme Length 303
Uniprot Accession Number P29031
Absorption
Active Site ACT_SITE 150; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:P29022
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14;
DNA Binding
EC Number 3.2.1.14
Enzyme Function FUNCTION: Defense against chitin-containing fungal pathogens.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Chain (1); Disulfide bond (5); Domain (1); Region (1); Signal peptide (1)
Keywords Carbohydrate metabolism;Chitin degradation;Chitin-binding;Disulfide bond;Glycosidase;Hydrolase;Plant defense;Polysaccharide degradation;Reference proteome;Signal
Interact With
Induction INDUCTION: By wounding.
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..21; /evidence=ECO:0000250
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 32,209
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda