| IED ID | IndEnz0009000195 |
| Enzyme Type ID | chitinase000195 |
| Protein Name |
Chitinase EC 3.2.1.14 Fragment |
| Gene Name | |
| Organism | Streptomyces violaceusniger |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Streptomycetales Streptomycetaceae Streptomyces Streptomyces violaceusniger group Streptomyces violaceusniger |
| Enzyme Sequence | GDGTGPGPGP |
| Enzyme Length | 10 |
| Uniprot Accession Number | P86977 |
| Absorption | |
| Active Site | |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by Hg(2+) and, to a lesser extent, by Ag(+). Hardly or not at all affected by Fe(3+), Mg(2+), Co(2+), Zn(2+), Ca(2+), K(+), EDTA, beta-mercaptoethanol and N-acetylglucosamine. {ECO:0000269|PubMed:22915152}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14; Evidence={ECO:0000269|PubMed:22915152}; |
| DNA Binding | |
| EC Number | 3.2.1.14 |
| Enzyme Function | FUNCTION: Has antifungal activity. Inhibits mycelial growth of wood rotting fungi P.chrysosporium MTCC 787, S.commune ITCC 3751, G.trabeum MTCC 355, P.agaricans ITCC 761, C.versicolor MTCC 138, P.versicolor ITCC 13, P.placenta MTCC 144 and P.friabilis ITCC 335. {ECO:0000269|PubMed:22915152}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 50 degrees Celsius. Retains only 60% and 25% activity after incubation at 30 degrees Celsius and 60 degrees Celsius for 1 hour, respectively. {ECO:0000269|PubMed:22915152}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 5 at 37 degrees Celsius. Retains >80% activity after incubation at pH 3-10 for 1 hour. {ECO:0000269|PubMed:22915152}; |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Non-terminal residue (1) |
| Keywords | Antimicrobial;Carbohydrate metabolism;Chitin degradation;Direct protein sequencing;Fungicide;Glycosidase;Hydrolase;Polysaccharide degradation;Secreted |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:22915152}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 811 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.143 mM for p-nitrophenyl-N-acetyl-beta-D-N,N'-diacetylchitobiose {ECO:0000269|PubMed:22915152}; Vmax=6.6 umol/h/mg enzyme with p-nitrophenyl-N-acetyl-beta-D-N,N'-diacetylchitobiose as substrate {ECO:0000269|PubMed:22915152}; |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |