| IED ID | IndEnz0009000207 |
| Enzyme Type ID | chitinase000207 |
| Protein Name |
Endochitinase 46 EC 3.2.1.14 46 kDa endochitinase Chitinase 46 |
| Gene Name | chit46 |
| Organism | Trichoderma harzianum (Hypocrea lixii) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Hypocreomycetidae Hypocreales Hypocreaceae Trichoderma Trichoderma harzianum (Hypocrea lixii) |
| Enzyme Sequence | MLSFLGKSVALLAALQATLSSASPLATEERSIEKRANGYANSVYFTNWGIYDRNFQPADLVASDVTHVIYSFMNLQADGTVVSGDTYADFEKHYADDSWNDVGTNAYGCAKQLFKVKKANRGLKVLLSIGGWTWSTNFPSAASTDANRKNFAKTAITFMKDWGFDGIDVDWEYPADATQASNMVLLLKEVRSQLDAYAAQYAPGYHFLLTIAAPAGKDNYSKLRLADLGQVLDYINLMAYDYAGSFSPLTGHDANLFANPSNPNATPFNTDSAVKDYIKGGVPANKIVLGMPIYGRSFQNTAGIGQTYNGVGGGGGGSTGSWEAGIWDYKALPKAGATIQYDSVAKGYYSYNAGTKELISFDTPDMINTKVAYLKSLGLGGSMFWEASADKKGADSLIGTSHRALGGLDTTQNLLSYPNSKYDNIRNGLN |
| Enzyme Length | 430 |
| Uniprot Accession Number | A6N6J0 |
| Absorption | |
| Active Site | ACT_SITE 172; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258 |
| Activity Regulation | |
| Binding Site | BINDING 173; /note=Chitooligosaccharide; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258; BINDING 385; /note=Chitooligosaccharide; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14; |
| DNA Binding | |
| EC Number | 3.2.1.14 |
| Enzyme Function | FUNCTION: Secreted chitinase involved in the degradation of chitin, a component of the cell walls of fungi and exoskeletal elements of some animals (including worms and arthropods). Plays a morphogenetic role during apical growth, cell division and differentiation (cell wall morphogenesis). Acts also as an antifungal agent. Involved in the degradation and further assimilation of phytopathogenic fungi, namely mycoparasitism, the major mechanism accounting for the antagonistic activity against phytopathogenic fungi displayed by Trichoderma. {ECO:0000269|PubMed:20213103}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Binding site (2); Chain (1); Domain (1); Glycosylation (1); Propeptide (1); Region (3); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Chitin degradation;Chitin-binding;Cleavage on pair of basic residues;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Secreted;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:20213103}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..22; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 46,380 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |