| IED ID | IndEnz0009000230 |
| Enzyme Type ID | chitinase000230 |
| Protein Name |
Endochitinase EC 3.2.1.14 Soluble cell wall protein 2 |
| Gene Name | CTS1 SCW2 YLR286C L8003.13 |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Enzyme Sequence | MSLLYIILLFTQFLLLPTDAFDRSANTNIAVYWGQNSAGTQESLATYCESSDADIFLLSFLNQFPTLGLNFANACSDTFSDGLLHCTQIAEDIETCQSLGKKVLLSLGGASGSYLFSDDSQAETFAQTLWDTFGEGTGASERPFDSAVVDGFDFDIENNNEVGYSALATKLRTLFAEGTKQYYLSAAPQCPYPDASVGDLLENADIDFAFIQFYNNYCSVSGQFNWDTWLTYAQTVSPNKNIKLFLGLPGSASAAGSGYISDTSLLESTIADIASSSSFGGIALWDASQAFSNELNGEPYVEILKNLLTSASQTATTTVATSKTSAASTSSASTSSASTSQKKTTQSTTSTQSKSKVTLSPTASSAIKTSITQTTKTLTSSTKTKSSLGTTTTESTLNSVAITSMKTTLSSQITSAALVTPQTTTTSIVSSAPIQTAITSTLSPATKSSSVVSLQTATTSTLSPTTTSTSSGSTSSGSTSSDSTARTLAKELNAQYAAGKLNGKSTCTEGEIACSADGKFAVCDHSAWVYMECASGTTCYAYDSGDSVYTQCNFSYLESNYF |
| Enzyme Length | 562 |
| Uniprot Accession Number | P29029 |
| Absorption | |
| Active Site | ACT_SITE 157; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14; |
| DNA Binding | |
| EC Number | 3.2.1.14 |
| Enzyme Function | FUNCTION: Chitinase is required for cell separation during growth of S.cerevisiae. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (16); Chain (1); Domain (1); Glycosylation (1); Helix (10); Natural variant (8); Region (3); Sequence conflict (2); Signal peptide (1); Turn (3) |
| Keywords | 3D-structure;Carbohydrate metabolism;Cell wall;Cell wall biogenesis/degradation;Chitin degradation;Chitin-binding;Direct protein sequencing;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:9748433}. Secreted, cell wall {ECO:0000269|PubMed:9748433}. Note=Most of the enzyme is secreted, but a significant amount of chitinase is also found associated with the cell wall through binding of C-terminal domain to chitin. |
| Modified Residue | |
| Post Translational Modification | PTM: Extensively glycosylated with a series of short O-linked mannose oligosaccharides ranging in size from Man(2) to Man(5). |
| Signal Peptide | SIGNAL 1..20; /evidence="ECO:0000269|PubMed:1918080, ECO:0000269|PubMed:9748433" |
| Structure 3D | X-ray crystallography (5) |
| Cross Reference PDB | 2UY2; 2UY3; 2UY4; 2UY5; 4TXE; |
| Mapped Pubmed ID | 10409653; 10517323; 10970846; 11259593; 11580836; 11747810; 12702273; 15256547; 15720543; 15856224; 1608450; 16249876; 16498706; 16760306; 1730413; 17524989; 17587671; 17898805; 17975704; 18076379; 18357467; 18687406; 19129178; 19596791; 20641019; 21072241; 22736599; 22977255; 23135325; 23212898; 23419716; 23447700; 23559820; 23959528; 23991176; 24145419; 24465507; 25063338; 26845196; 26975390; 27402737; 28034796; 3033651; 6799506; 8657150; 9745020; 9763195; |
| Motif | |
| Gene Encoded By | |
| Mass | 59,015 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.2.1.14; |