| IED ID | IndEnz0009000243 |
| Enzyme Type ID | chitinase000243 |
| Protein Name |
Chitinase 2 EC 3.2.1.14 Class I chitinase b OsChia1b Pathogenesis related PR -3 chitinase 2 |
| Gene Name | Cht2 RC7 Os05g0399300 LOC_Os05g33130 OsJ_18467 |
| Organism | Oryza sativa subsp. japonica (Rice) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. japonica (Rice) |
| Enzyme Sequence | MSTPRAAASLAKKAALVALAVLAAALATAARAEQCGAQAGGARCPNCLCCSRWGWCGTTSDFCGDGCQSQCSGCGPTPTPTPPSPSDGVGSIVPRDLFERLLLHRNDGACPARGFYTYEAFLAAAAAFPAFGGTGNTETRKREVAAFLGQTSHETTGGWPTAPDGPFSWGYCFKQEQNPPSDYCQPSPEWPCAPGRKYYGRGPIQLSFNFNYGPAGRAIGVDLLSNPDLVATDATVSFKTALWFWMTPQGNKPSSHDVITGRWAPSPADAAAGRAPGYGVITNIVNGGLECGHGPDDRVANRIGFYQRYCGAFGIGTGGNLDCYNQRPFNSGSSVGLAEQ |
| Enzyme Length | 340 |
| Uniprot Accession Number | Q7DNA1 |
| Absorption | |
| Active Site | ACT_SITE 154; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:P29022 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14; |
| DNA Binding | |
| EC Number | 3.2.1.14 |
| Enzyme Function | FUNCTION: Hydrolyzes chitin and plays a role in defense against fungal pathogens containing chitin. Its overexpression confers enhanced resistance to sheath blight pathogen (R.solani). {ECO:0000269|PubMed:11166426, ECO:0000269|PubMed:18211919, ECO:0000269|PubMed:18323646}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (4); Chain (1); Disulfide bond (8); Domain (1); Erroneous gene model prediction (1); Helix (14); Mutagenesis (1); Signal peptide (1); Turn (6) |
| Keywords | 3D-structure;Carbohydrate metabolism;Chitin degradation;Chitin-binding;Disulfide bond;Glycosidase;Hydrolase;Plant defense;Polysaccharide degradation;Reference proteome;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..32; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (2) |
| Cross Reference PDB | 2DKV; 3IWR; |
| Mapped Pubmed ID | 20544965; |
| Motif | |
| Gene Encoded By | |
| Mass | 35,587 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |