| IED ID | IndEnz0009000257 |
| Enzyme Type ID | chitinase000257 |
| Protein Name |
Chitinase A CHI-A EC 3.2.1.14 |
| Gene Name | chiA |
| Organism | Pseudoalteromonas piscicida |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Alteromonadales Pseudoalteromonadaceae Pseudoalteromonas Pseudoalteromonas piscicida |
| Enzyme Sequence | MKLNKITSYIGFALLSGGALAAPSTPTLDWQPQQYSFVEVNVDGLGSYKQLVKAKDVVDISIKWNAWSGSGGDNYKVYFDDLLVNQGSLPAGTKSGVVQFPYTKSGRHQLYLELCEGTVCARSAGKEIVIADTDGAHLAPLPMNVDPNNRNNGTIPGRVTGAYFVEWGIYGRNYDVTKIPAHNLSHILYGFIPICGPNESLKSIEIGNSWRALQTACADSQDYEVVIHDPWAAVQKSMPGVDAKDPIRGVYSQLMALKQRYPDLKILPSVGGWTLSDPFHGFTNKANRDTFVASVKQFLKTWKFYDGVDIDWEFPGGDGPNPDLGDPINDGPAYVALMQELRAMLDELEAETGRQYELTSAIGAGYDKIEDVDYQAAQQYMDYIFAMTYDFYGAWNNETGHQTGIYCGSHLSTDECNGTGVDDNGVPRKGPAYTGDHAIQLLLQQGVQPSKLVMGVAMYGRGWEGVLDANAAIPGNPMTAPGNGPLTGSTSEGVWEPGIMDYKAIAANAVGQGGSGVNGYEVGYDEQAQAAYVWNRSNGKLITYDSPRSVIAKGQYANTHQLAGLFGWEIDADNGDILNAMYDGLTAGEIPNRAPTIGVSGPINVTSGQVVNVDAQASDLDNDPLTYSWVAAPGLALSANNTAAVAVTAPSVAQQTSYDLTVTVNDGALSTTKTIVVVVNPEGANAAPVVTPVSDISVNEGASATVNVSATDPEGAALSYSWSVPAELSVANGSSATITAANVTADTTVPVTVTVSDGVNAVDTTFNVTIKDGAEYPTWDRSTVYVGGDRVIHNSNVFEAKWWTQGEEPGTADVWKAVTN |
| Enzyme Length | 820 |
| Uniprot Accession Number | P32823 |
| Absorption | |
| Active Site | ACT_SITE 313; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258 |
| Activity Regulation | ACTIVITY REGULATION: Stimulated by magnesium ions; inhibited by N-bromosuccinimide and 2-hydroxy-5-nitrobenzyl bromide. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14; |
| DNA Binding | |
| EC Number | 3.2.1.14 |
| Enzyme Function | |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 50 degrees Celsius.; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 8.0.; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Domain (1); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Chitin degradation;Direct protein sequencing;Glycosidase;Hydrolase;Polysaccharide degradation;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..21; /evidence=ECO:0000269|PubMed:1464065 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 87,346 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |