| IED ID | IndEnz0009000261 |
| Enzyme Type ID | chitinase000261 |
| Protein Name |
Endochitinase EC 3.2.1.14 Allergen Pers a 1 Allergen Prs a 1 Chitin-binding avocado protein CBAP Class I chitinase PaI1 allergen Pers a 1.0101 |
| Gene Name | chi1 |
| Organism | Persea americana (Avocado) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Magnoliidae Laurales Lauraceae Persea Persea americana (Avocado) |
| Enzyme Sequence | MVYCTASLPLLLLLLVGLLAGEAFAEQCGRQAGGALCPGGLCCSQFGWCGSTSDYCGPTCQSQCGGVTPSPGGGVASLISQSVFNQMLKHRNDAACQAKGFYTYNAFIAAANSFNGFASVGDTATRKREIAAFLAQTSHETTGGWATAPDGPYAWGYCFLKEQGNPPDYCVPTAQWPCAPGKKYYGRGPIQISYNYNYGPAGRAIGYDLINNPDAVATDPVISFKTALWFWMTPQSPKPSCHNVITGRWTPSAADRAAGRLPGYGVITNIINGGIECGKGFNDKVADRIGFYKRYCDLLGVSYGSNLDCYNQRSFGVSTNPLAASS |
| Enzyme Length | 326 |
| Uniprot Accession Number | P93680 |
| Absorption | |
| Active Site | ACT_SITE 140; /note=Proton donor; /evidence=ECO:0000255|PIRSR:PIRSR001060-1 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14; Evidence={ECO:0000269|PubMed:9679856, ECO:0000269|PubMed:9774427}; |
| DNA Binding | |
| EC Number | 3.2.1.14 |
| Enzyme Function | FUNCTION: Defense against chitin-containing fungal pathogens. Has in vitro antifungal activity against F.oxysporum inhibiting its growth and the branching of its hyphae. Has endochitinase activity, but no exochitinase or lysozyme activities. {ECO:0000269|PubMed:9774427}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Disulfide bond (7); Domain (1); Sequence conflict (1); Signal peptide (1) |
| Keywords | Allergen;Antimicrobial;Carbohydrate metabolism;Chitin degradation;Chitin-binding;Direct protein sequencing;Disulfide bond;Glycosidase;Hydrolase;Pathogenesis-related protein;Plant defense;Polysaccharide degradation;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..25; /evidence="ECO:0000255, ECO:0000269|PubMed:10231327, ECO:0000269|PubMed:14610495, ECO:0000269|PubMed:9679856, ECO:0000269|PubMed:9774427" |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 34,586 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |