| IED ID | IndEnz0009000272 |
| Enzyme Type ID | chitinase000272 |
| Protein Name |
Viral cathepsin V-cath EC 3.4.22.50 Cysteine proteinase CP |
| Gene Name | VCATH ORF127 |
| Organism | Autographa californica nuclear polyhedrosis virus (AcMNPV) |
| Taxonomic Lineage | Viruses Naldaviricetes Lefavirales Baculoviridae Alphabaculovirus Autographa californica multiple nucleopolyhedrovirus Autographa californica nuclear polyhedrosis virus (AcMNPV) |
| Enzyme Sequence | MNKILFYLFVYGVVNSAAYDLLKAPNYFEEFVHRFNKDYGSEVEKLRRFKIFQHNLNEIINKNQNDSAKYEINKFSDLSKDETIAKYTGLSLPIQTQNFCKVIVLDQPPGKGPLEFDWRRLNKVTSVKNQGMCGACWAFATLASLESQFAIKHNQLINLSEQQMIDCDFVDAGCNGGLLHTAFEAIIKMGGVQLESDYPYEADNNNCRMNSNKFLVQVKDCYRYITVYEEKLKDLLRLVGPIPMAIDAADIVNYKQGIIKYCFNSGLNHAVLLVGYGVENNIPYWTFKNTWGTDWGEDGFFRVQQNINACGMRNELASTAVIY |
| Enzyme Length | 323 |
| Uniprot Accession Number | P25783 |
| Absorption | |
| Active Site | ACT_SITE 136; /evidence=ECO:0000255|PROSITE-ProRule:PRU10088; ACT_SITE 269; /evidence=ECO:0000255|PROSITE-ProRule:PRU10089; ACT_SITE 289; /evidence=ECO:0000255|PROSITE-ProRule:PRU10090 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endopeptidase of broad specificity, hydrolyzing substrates of both cathepsin L and cathepsin B.; EC=3.4.22.50; |
| DNA Binding | |
| EC Number | 3.4.22.50 |
| Enzyme Function | FUNCTION: Cysteine protease that plays an essential role in host liquefaction to facilitate horizontal transmission of the virus. Accumulates within infected cells as an inactive proenzyme (proV-CATH), which is activated by proteolytic cleavage upon cell death. {ECO:0000269|PubMed:12069520}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 5.0-5.5.; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (3); Erroneous initiation (1); Glycosylation (1); Propeptide (1); Signal peptide (1) |
| Keywords | Disulfide bond;Glycoprotein;Host endoplasmic reticulum;Hydrolase;Protease;Reference proteome;Signal;Thiol protease;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Host endoplasmic reticulum {ECO:0000269|PubMed:21289117}. Note=Retained in the host reticulum by its interaction with chitinase/CHIA. {ECO:0000269|PubMed:21289117}. |
| Modified Residue | |
| Post Translational Modification | PTM: Synthesized as an inactive proenzyme and activated by proteolytic removal of the inhibitory propeptide. {ECO:0000269|PubMed:12069520}. |
| Signal Peptide | SIGNAL 1..16; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 36,938 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.4.22.50; |